Strain Information | |
---|---|
Image | |
BRC No. | RBRC04799 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6;B6129-EpCAM<tm1.1Hmd> |
Former Common name | EpCAM floxed |
H-2 Haplotype | |
ES Cell line | |
Background strain | |
Appearance | |
Strain development | Developed by Dr. Hiroshi Hamada, Graduate School of Frontier Biosciences, Osaka University. The construct was electroporated into G4 ES cells derived from B6129F1. The SA-IRES-Bgeo was excised by crossing with mice expressing F1p (B6-FLPe transgenic mice). |
Strain description | Exon2 and exon3 of Tacstd1 (Epcam, epithelial cell adhesion molecule) gene flanked by loxp sites. Conditional Tacstd1 deficient mice can be generated by cyossing with tissue-specific Cre mice. Homozygous mice were viable and fertile. |
Colony maintenance | Heterozygote X Wild-type [C57BL/6JJcl] |
References | Dev. Biol., 371, 136-145 (2012). 22819673 |
Health Report | |
---|---|
Examination Date / Room / Rack |
Gene | |
---|---|
Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site17Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP17loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP17loxP |
Ordering Information | |
---|---|
Donor DNA | phage P1 loxP sites, yeast FRT (flippase recombination target) site, mouse Epcam genomic DNA |
Research application | Cre/loxP system FLP/frt system |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Dev. Biol., 371, 136-145 (2012). |
Depositor | Hiroshi Hamada (RIKEN CDB) |
Strain Status | Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
---|
No Data |