Strain Data Sheet
Data update: Jun 20, 2019
Health Report   PCR Protocol   Thawing Method
embryos / sperm >
  Mouse Marking
RBRC No. RBRC04832  
Type Targeted MutationCartagena  
Species Mus musculus  
Strain name B6.129P2(CD1)-Id4<tm1Fsky>  
Former Common name B6-Id4 KO  
H-2 Haplotype No Data  
ES cell line E14.1 [129P2/OlaHsd]  
Background strain No Data  
 1 Appearance No Data  
Genotype No Data  
Strain development Developed by Fred Sblitzky, University of Nottingham in 2005. A lacZ-neo cassette replaced 220 bp of the ID4 cording region including the HLH domain. The mutant mice were backcrossed to C57BL/6 from 129 and CD1 (ICR) mixed background.  
Strain description Id4 knockout mice. Id4 is a member of inhibitor of differentiation / inhibitor of DNA binding protein family. Homozygous mutant mice exhibit a abnormal brain structures.  
Colony maintenance Heterozygote X Wild-type [C57BL/6JJcl] Homozygous mutant mice are small and most of them die within 8 weeks of age.  
Health Report No Data  
Gene Details
Promoter No Data  
 1 Symbol Id4  
Symbol name inhibitor of DNA binding 4  
Chromosome 13  
Common name No Data  
Symbol description No Data  
 2 Symbol lacZ  
Symbol name beta-galactosidase (E. coli)  
Chromosome No Data  
Common name No Data  
Symbol description E. coli beta galactosidase  
Promoter herpes simplex virus thymidine kinase promoter (HSV tk promoter)  
 3 Symbol neo  
Symbol name neomycin resistance gene (E. coli)  
Chromosome No Data  
Common name neo; neomycin;  
Symbol description No Data  
Research applications Fluorescent Proteins/lacZ System  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Dev. Biol., 280, 386-395 (2005).
The RECIPIENT must contact Prof. Fred. Sablitzky, The University of Nottingham ( to obtain permission for us the BIOLOGICAL RESOURCE.  
Additional information
 1 No Data  
 2 Genotyping protocol <PCR>  
Depositor Okazaki, Yasuji (Saitama medical school research center for genomic medicine)  Okazaki, Yasuji 
Strain Status /
(Expected delivery)

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