Strain Data Sheet

RBRC04942

Strain Information

Image
BRC No.RBRC04942
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129-Cntn2<tm1Fuka>
Former Common nameTAG-1 KO
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strain
Appearance
Strain developmentDeveloped by Kazutada Watanabe, Nagaoka University of Technology in 2001. The region between exon 2 and 5, encoding the translational start codon through the first Ig-like domain was replaced with a neo cassette.
Strain description
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJmsSlc]
ReferencesBiochem. Biophys. Res. Commun., 281, 220-226 (2001). 11178983
J. Cell Biol., 162, 1161-1172 (2003). 12975355

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Cntn2contactin 21Cntn2<tm1Fuka>targeted mutation 1, Fumihiko Fukamauchi

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)1neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter)

Ordering Information

Donor DNAMouse TAG-1 (Cntn2) genomic DNA, E. coli Neomycin resistance gene
Research application
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Biochem. Biophys. Res. Commun., 281, 220-226 (2001). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEVELOPER, Dr. Kazutada Watanabe is requested.
DepositorYasushi Shimoda (Nagaoka University of Technology)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data