Strain Information | |
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Image | |
BRC No. | RBRC05145 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6.129P2-Prdm9<tm1Ymat> |
Former Common name | B6-Meisetz KO |
H-2 Haplotype | |
ES Cell line | E14.1 [129P2/OlaHsd] |
Background strain | |
Appearance | |
Strain development | Developed by Katsuhiko Hayashi and Yasuhisa Matsui, Osaka Medical Center for Maternal and Child Health in 1995. A targeting vector containing the NLS-lacZ and a loxP-flanked PGFneo cassette was inserted into the initiation codon of exon 2, all of exons 3 to 6, and part of exon 7. The mutant mice were backcrossed to C57BL/6JJcl. |
Strain description | Meisetz (Prdm9) knockout mice. Homozygous mutant mice show meiotic arrest and both female and male are infertile. |
Colony maintenance | Heterozygote x Wild-type [C57BL/6JJmsSlc] |
References | Nature, 438, 374-378 (2005). 16292313 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter lacZbeta-galactosidase (E. coli)17 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP17loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP17loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)17mouse phosphoglycerate kinase promoter (PGK promoter) Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SV40 PolyA signal17 |
Ordering Information | |
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Donor DNA | Simian virus 40 Large T antigen nuclear localization signal (NLS), E. coli Beta galactosidase gene, SV40 PolyA signal, Phage P1 loxP sites, mouse phosphoglycerate kinase promoter, E. coli Neomycin resistance gene, mouse Prdm9 genomic DNA |
Research application | Cre/loxP system Fluorescent Proteins/lacZ System |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nature. 438, 374-378 (2005).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. |
Depositor | Yasuhisa Matsui (Tohoku University) |
Strain Status | Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |