Strain Data Sheet
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Data update: Jun 20, 2019
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RBRC No. RBRC05145  
Type Targeted MutationCartagena  
Species Mus musculus  
Strain name B6.129P2-Prdm9<tm1Ymat>  
Former Common name B6-Meisetz KO  
H-2 Haplotype No Data  
ES cell line E14.1 [129P2/OlaHsd]  
Background strain No Data  
Appearance
 1 Appearance No Data  
Genotype No Data  
Strain development Developed by Katsuhiko Hayashi and Yasuhisa Matsui, Osaka Medical Center for Maternal and Child Health in 1995. A targeting vector containing the NLS-lacZ and a loxP-flanked PGFneo cassette was inserted into the initiation codon of exon 2, all of exons 3 to 6, and part of exon 7. The mutant mice were backcrossed to C57BL/6JJcl.  
Strain description Meisetz (Prdm9) knockout mice. Homozygous mutant mice show meiotic arrest and both female and male are infertile.  
Colony maintenance Heterozygote x Wild-type [C57BL/6JJmsSlc]  
Health Report
Gene Details
Promoter No Data  
 1 Symbol Prdm9  
Symbol name PR domain containing 9  
Chromosome 17  
Common name No Data  
Symbol description No Data  
 2 Symbol No Data  
Symbol name SV40 PolyA signal  
Chromosome 17  
Common name No Data  
Symbol description No Data  
 3 Symbol lacZ  
Symbol name beta-galactosidase (E. coli)  
Chromosome 17  
Common name No Data  
Symbol description No Data  
 4 Symbol loxP  
Symbol name phage P1 loxP  
Chromosome 17  
Common name No Data  
Symbol description No Data  
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)  
 5 Symbol neo  
Symbol name neomycin resistance gene (E. coli)  
Chromosome 17  
Common name No Data  
Symbol description No Data  
References Nature 2005 Nov 17;438(7066):374-8.  
A histone H3 methyltransferase controls epigenetic events required for meiotic prophase.
Research applications Cre/loxP system,
Fluorescent Proteins/lacZ System  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Nature. 438, 374-378 (2005).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.  
Additional information
 1 No Data  
 2 Genotyping protocol <PCR>  
Depositor Matsui, Yasuhisa (Institute of Development, Aging and Cancer, Tohoku University)  Matsui, Yasuhisa 
Strain Status /
Availability
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