Strain Data Sheet
ANIMAL SEARCH SYSTEM
Data update: Aug 20, 2019
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RBRC No. RBRC05152  
Type Targeted MutationCartagena  
Species Mus musculus  
Strain name B6;129S6-Gt(ROSA)26Sor<tm1.2(CAG-eNpHR2/EYFP)Imayo>  
Former Common name R26-CAG-FRT-eNpHR2-EYFP  
H-2 Haplotype No Data  
ES cell line TC1 [129S6/SvEvTac]  
Background strain No Data  
Appearance
 1 Appearance No Data  
Genotype No Data  
Strain description eNpHR2-EYFP is expressed after FLP-recombination. The transgene construct is knocked into Rosa26-locus. To achieve high-level transgene expression, eNpHR2-EYFP is expressed under CAG promoter and WPRE sequence is inserted into 3'UTR region.  
Health Report No Data  
Gene Details
Promoter No Data  
 1 Symbol Gt(ROSA)26Sor  
Symbol name gene trap ROSA 26, Philippe Soriano  
Chromosome 6  
Common name No Data  
Symbol description No Data  
 2 Symbol EYFP  
Symbol name Enhanced Yellow Fluorescent Protein (Jellyfish)  
Chromosome 6  
Common name No Data  
Symbol description No Data  
 3 Symbol Frt  
Symbol name yeast FRT (flippase recombination target) site  
Chromosome 6  
Common name No Data  
Symbol description No Data  
 4 Symbol Frt  
Symbol name yeast FRT (flippase recombination target) site  
Chromosome 6  
Common name No Data  
Symbol description No Data  
 5 Symbol WPRE  
Symbol name Woodchuck hepatitis virus posy-transcriptional regulatory element  
Chromosome 6  
Common name No Data  
Symbol description No Data  
 6 Symbol eNpHR2  
Symbol name Natronomonas enhanced halorhodopsin 2  
Chromosome 6  
Common name No Data  
Symbol description No Data  
 7 Symbol loxP  
Symbol name phage P1 loxP  
Chromosome 6  
Common name No Data  
Symbol description No Data  
 8 Symbol poly A  
Symbol name poly A  
Chromosome 6  
Common name No Data  
Symbol description No Data  
 9 Symbol puro  
Symbol name puromycin resistance gene (Streptomyces alboniger)  
Chromosome 6  
Common name No Data  
Symbol description No Data  
References   
Research applications Cre/loxP system,
FLP/frt system,
Fluorescent Proteins/lacZ System  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Neurosci. Res., 75(1):53-58 (2013).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT of BIOLOGICAL resource shall obtain a prior written consent on use of it from the DEPOSITOR Dr. Itaru Imayoshi (E-mail: iimayosh@virus.kyoto-u.ac.jp). (No approval form (Form D) is necessary). The RECIPIENT of the BIOLOGICAL RESOURCE is requested to make a Material Transfer Agreement specified by the DEPOSITOR.  
Additional information
 1 Dr. Karl Deisseroth, MTA
GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.
Itaru Imayoshi HP  
 2 Genotyping protocol <PCR>  
Depositor Imayoshi, Itaru (Institute for Virus Research, Kyoto University)  Imayoshi, Itaru 
Strain Status /
Availability
(Expected delivery)

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