Strain Data Sheet
ANIMAL SEARCH SYSTEM
Data update: May 20, 2019
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RBRC No. RBRC05158  
Type Targeted MutationCartagena  
Species Mus musculus  
Strain name B6;129S6-Gt(ROSA)26Sor<tm1.2(CAG-ChR2/mCherry)Imayo>  
Former Common name R26-CAG-FRT-ChR2(C128A)-mCherry  
H-2 Haplotype No Data  
ES cell line TC1 [129S6/SvEvTac]  
Background strain No Data  
Appearance
 1 Appearance No Data  
Genotype No Data  
Strain description ChR2 (C128A) -mCherry is expressed after FLP-recombination. The transgene construct is knocked into Rosa26-locus. To achieve high-level transgene expression, ChR2 (C128A) -mCherry is expressed under CAG promoter and WPRE sequence is inserted into 3'UTR region.  
Health Report No Data  
Gene Details
Promoter No Data  
 1 Symbol Gt(ROSA)26Sor  
Symbol name gene trap ROSA 26, Philippe Soriano  
Chromosome 6  
Common name No Data  
Symbol description No Data  
 2 Symbol No Data  
Symbol name HSV thymidine kinase polyA signal  
Chromosome 6  
Common name No Data  
Symbol description No Data  
 3 Symbol No Data  
Symbol name SV40 early polyA signal  
Chromosome 6  
Common name No Data  
Symbol description No Data  
 4 Symbol No Data  
Symbol name SV40 late polyA signal  
Chromosome 6  
Common name No Data  
Symbol description No Data  
 5 Symbol Frt  
Symbol name yeast FRT (flippase recombination target) site  
Chromosome 6  
Common name No Data  
Symbol description No Data  
 6 Symbol GH  
Symbol name growth hormone polyadenylation signal  
Chromosome 6  
Common name No Data  
Symbol description No Data  
 7 Symbol WPRE  
Symbol name Woodchuck hepatitis virus posy-transcriptional regulatory element (WPRE)  
Chromosome 6  
Common name No Data  
Symbol description No Data  
 8 Symbol loxP  
Symbol name phage P1 loxP  
Chromosome 6  
Common name No Data  
Symbol description No Data  
 9 Symbol mCherry  
Symbol name Red Fluorescent Protein (Discosoma sp.)  
Chromosome 6  
Common name No Data  
Symbol description No Data  
 10 Symbol puro  
Symbol name puromycin resistance gene (Streptomyces alboniger)  
Chromosome 6  
Common name No Data  
Symbol description No Data  
Promoter CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)  
 11 Symbol ChR2 (C128A)  
Symbol name Chlamydomonas Channelrhodopsin-2 mutant C128A  
Chromosome 6  
Common name No Data  
Symbol description No Data  
References   
Research applications No Data  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Neurosci. Res., 75(1):53-58 (2013).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT of BIOLOGICAL resource shall obtain a prior written consent on use of it from the DEPOSITOR Dr. Itaru Imayoshi (E-mail: iimayosh@virus.kyoto-u.ac.jp). (No approval form (Form D) is necessary). The RECIPIENT of the BIOLOGICAL RESOURCE is requested to make a Material Transfer Agreement specified by the DEPOSITOR.  
Additional information
 1 Dr. Karl Deisseroth, MTA
Itaru Imayoshi HP  
Depositor Imayoshi, Itaru (Institute for Virus Research, Kyoto University)  Imayoshi, Itaru 
Strain Status /
Availability
(Expected delivery)

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