Strain Information | |
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Image | |
BRC No. | RBRC05243 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6;129B6-Pitx2<tm2.1Hmd> |
Former Common name | B6-Pitx2c/lacZ KI |
H-2 Haplotype | |
ES Cell line | G4 [(129S6/SvEvTac x C57BL/6NCr)F1] |
Background strain | |
Appearance | |
Strain development | Developed by Hidetaka Shiratori and Hiroshi Hamada, Institute for Molecular and Cellular Biology, Osaka University in 2010. G4 ES cells derived from (129S6/SvEvTac x C57BL/6NCr)F1 were used to generate the mutant mice. A frt flanked neo cassette was removed by crossing with CAG-FLPe mice. |
Strain description | Pitx2c knockout mice (lacZ reporter). Details unknown. |
Colony maintenance | Heterozygote x Wild-type [C57BL/6JJcl] |
References | Dev. Biol., 395(2):331-41 (2014). 25224222 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Pitx2paired-like homeodomain transcription factor 23Pitx2<tm2.1Hmd>targeted mutation 2.1, Hiroshi Hamada Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site3Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter lacZbeta-galactosidase (E. coli)3 |
Ordering Information | |
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Donor DNA | E. coli beta-galactosidase, yeast FRT (flippase recombination target) site, mouse Pitx2c genomic DNA |
Research application | FLP/frt system Fluorescent Proteins/lacZ System |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Dev. Biol., 395(2):331-41 (2014). |
Depositor | Hiroshi Hamada (RIKEN CDB) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |