Strain Data Sheet
ANIMAL SEARCH SYSTEM
Data update: Aug 20, 2019
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RBRC No. RBRC05249  
Type TransgeneCartagena  
Species Mus musculus  
Strain name B6;B6D2-Tg(Pou2f3-Ascl1-ires-nEGFP)1Abek  
Former Common name B6-Pou2f3-Ascl1/GFP Tg#1  
H-2 Haplotype No Data  
Background strain No Data  
Appearance
 1 Appearance No Data  
Genotype No Data  
Strain development Developed by Ichiro Matsumoto (Graduate School of Agricultural and Life Sciences, The University of Tokyo / Monell Chemical Senses Center) and Keiko Abe (Graduate School of Agricultural and Life Sciences, The University of Tokyo). The transgene was injected into the pronuclei of B6D2F1 fertilized eggs.  
Strain description These transgenic mice express Ascl1 and nGFP genes under the control of the Pou2f3 promoter.  
Colony maintenance Carrier x Noncarrier [C57BL/6JJcl]  
Health Report No Data  
Gene Details
Promoter No Data  
 1 Symbol Ascl1  
Symbol name achaete-scute complex homolog-like 1 (Drosophila)  
Chromosome UN  
Common name No Data  
Symbol description No Data  
 2 Symbol EGFP  
Symbol name Enhanced Green Fluorescent Protein, F64L and S65T (Jellyfish)  
Chromosome UN  
Common name No Data  
Symbol description No Data  
 3 Symbol IRES  
Symbol name internal ribosomal entry site (EMCV)  
Chromosome UN  
Common name No Data  
Symbol description No Data  
 4 Symbol nls  
Symbol name SV40 large T antigen nuclear localization signal (NLS)  
Chromosome UN  
Common name No Data  
Symbol description No Data  
References No Data  
Research applications Fluorescent Proteins/lacZ System  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENIT of BIOLOGICAL RESOURCE must notify the content of the research to the DEPOSITOR and obtain a prior written consent on use of it from the DEPOSITOR. (Ichiro Matsumoto: ichiro.matsumoto1@gmail.com). The RECIPIENT of BIOLOGICAL RESOURCE agrees to include the DEPOSITOR and its colleagues as co-authors in first publication of the results obtained by use of the B|OLOGICAL RESOURCE.  
Additional information
 1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.  
 2 Genotyping protocol <PCR>  
Depositor Matsumoto, Ichiro (Monell Chemical Senses Center)  Matsumoto, Ichiro 
Strain Status /
Availability
(Expected delivery)

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