Strain Information | |
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Image | |
BRC No. | RBRC05250 |
Type | Transgene |
Species | Mus musculus |
Strain name | B6;B6D2-Tg(Pou2f3-Ascl1-ires-nEGFP)2Abek |
Former Common name | B6-Pou2f3-Ascl1/GFP Tg#2 |
H-2 Haplotype | |
ES Cell line | |
Background strain | |
Appearance | |
Strain development | Developed by Ichiro Matsumoto (Graduate School of Agricultural and Life Sciences, The University of Tokyo / Monell Chemical Senses Center) and Keiko Abe (Graduate School of Agricultural and Life Sciences, The University of Tokyo). The transgene was injected into the pronuclei of B6D2F1 fertilized eggs. |
Strain description | These transgenic mice express Ascl1 and nGFP genes under the control of the Pou2f3 promoter. |
Colony maintenance | Carrier x Noncarrier [C57BL/6JJcl] |
References |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter EGFPEnhanced Green Fluorescent Protein (Aequorea victoria)UNEGFP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter IRESinternal ribosomal entry site (EMCV)UN Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter nlsSV40 large T antigen nuclear localization signal (NLS)UNnls Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter PolyA signalUN |
Ordering Information | |
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Donor DNA | Pou2f3 genomic DNA, Ascl1 cDNA, ires, large T antigen Nuclear Localization Signal, GFP cDNA, PolyA signal |
Research application | Fluorescent Proteins/lacZ System |
Specific Term and Conditions | The RECIPIENIT of BIOLOGICAL RESOURCE must notify the content of the research to the DEPOSITOR and obtain a prior written consent on use of it from the DEPOSITOR. (Ichiro Matsumoto: ichiro.matsumoto1@gmail.com). The RECIPIENT of BIOLOGICAL RESOURCE agrees to include the DEPOSITOR and its colleagues as co-authors in first publication of the results obtained by use of the B|OLOGICAL RESOURCE. |
Depositor | Ichiro Matsumoto (Monell Chemical Senses Center) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |