Strain Data Sheet

RBRC05269

Strain Information

Image
BRC No.RBRC05269
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Mir9-2<tm1Sia>/Rbrc
Former Common namemicroRNA9-2 (Acc. No. CDB0072K)
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeveloped by Shinichi Aizawa, RIKEN Center for Developmental Biology.
Strain descriptionmicroRNA9-2 knockout mice. Homozygous mutant males die within 1 month after birth.
Colony maintenanceHeterozygote x Wild-type [C57BL/6N]
ReferencesJ. Neurosci., 31, 3407-3422 (2011). 21368052

Health Report

Examination Date / Room / Rack2023/03/13Room:3-5Rack:C
2022/12/12Room:3-5Rack:C
2022/09/12Room:3-5Rack:C
2022/06/13Room:3-5Rack:C

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Mir9-2microRNA 9-213Mir9-2<tm1Sia>targeted mutation 1, Shinichi Aizawa

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP13loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP13loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)13neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 polyA signal13

Ordering Information

Donor DNABacteriophage-P1 loxP sites, Mouse (PGK promoter, PGK 3' end), E. coli Neo, simian virus 40 (SV40) poly(A) signal, mouse Mir9-2 genomic DNA
Research applicationCre/loxP system
Specific Term and Conditions(1) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the Acc.No. CDB0072K and Web. Site (https://large.riken.jp/distribution/mutant-list.html) is requested. (2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designed by the DEPOSITOR is requested. (J Neurosci. 2011 31(9):3407-22.) (3) Please inform the DEPOSITOR (mutant.bdr@riken.jp) about the paper when published.
DepositorRIKEN CDB (RIKEN CDB)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data