Strain Data Sheet

RBRC05273

Strain Information

Image
BRC No.RBRC05273
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Prickle1<tm1Nue>/Rbrc
Former Common namempk1 (Acc. No. CDB0431K)
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeveloped by Naoto Ueno, National Institute for Basic Biology.
Strain descriptionPrickle1, prickle homolog 1 (Drosophila) Knockout mice (mpk1(lacZ)). Homozygous mutant mice die between E7.5 and E8.5. Heterozygous mutant mice show decreased seizure threshold compared with wild-type.
Colony maintenanceHeterozygote x Wild-type [C57BL/6N]
ReferencesProc. Natl. Acad. Sci. USA, 106, 14426-14431 (2009). 19706528

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Prickle1prickle homolog 1 (Drosophila)15Prickle1<tm1Nue>targeted mutation 1, Naoto Ueno

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site15Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lacZbeta-galactosidase (E. coli)15

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lox71mutant lox sequence15lox71

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP15loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)15neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter)

Ordering Information

Donor DNALacZ, Neo : 大腸菌由来 / loxP, lox71 : P1ファージ由来 / frt : 酵母由来 / rabbit beta-globin 3’ end : ウサギ由来, Mouse PGK promoter
Research application
Specific Term and Conditions(1) The RECIPIENT shall obtain a prior agreement from the DEVELOPER (Dr. Naoto Ueno, National Institute for Basic Biology, E-mail: nueno@nibb.ac.jp) to transfer the BIOLOGICAL RESOURCE. (2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the Acc.No. CDB0431K and Web. Site (https://large.riken.jp/distribution/mutant-list.html) is requested. (3) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designed by the DEPOSITOR is requested. (Proc Natl Acad Sci U S A. 2009 106(34):14426-31.) (4) Please inform the DEPOSITOR (mutant.bdr@riken.jp) about the paper when published.
DepositorRIKEN CDB (RIKEN CDB)
Strain Statusan icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data