Strain Data Sheet

RBRC05276

Strain Information

Image
BRC No.RBRC05276
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameKIF-k (Acc. No.CDB0440K)
Former Common nameKIF-k (Acc. No.CDB0440K)
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Ryuichi Nishinakamura, Institute of Molecular Embryology and Genetics, Kumamoto University.
Strain descriptionKif26b, kinesin family member 26B knockout mice (KIF-k(lacZ)). Homozygous mice die within 24hrs after birth.
Colony maintenanceHeterozygote x Wild-type [C57BL/6N]
ReferencesProc. Natl. Acad. Sci. USA, 107, 9240-9245 (2010). 20439720

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Kif26bkinesin family member 26B1Kif26b <tm1Ryn>targeted mutation 1, Ryuichi Nishinakamura

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site1Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site1Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lacZbeta-galactosidase (E. coli)1

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lox71mutant lox sequence1lox71

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP1loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)1neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter)

Ordering Information

Donor DNAphage P1 lox71 site, E. coli lacZ, polyA, yeast FRT (flipase recombination target) sites, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse PGK3’end, phage P1 loxP site, mouse Kif26b genomic DNA
Research applicationCre/loxP system
FLP/frt system
Fluorescent Proteins/lacZ System
Specific Term and Conditions(1) The RECIPIENT shall obtain a prior agreement from the DEVELOPER (Dr. Ryuichi Nishinakamura, Kumamoto University, E-mail: ryuichi@gpo.kumamoto-u.ac.jp) to transfer the BIOLOGICAL RESOURCE. (2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the Acc.No. CDB0440K and Web. Site (https://large.riken.jp/distribution/mutant-list.html) is requested. (3) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designed by the DEPOSITOR is requested. (Proc Natl Acad Sci U S A. 2010 107(20):9240-5.) (4) Please inform the DEPOSITOR (mutant.bdr@riken.jp) about the paper when published.
DepositorRIKEN CDB (RIKEN CDB)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data