Strain Data Sheet

RBRC05285

Strain Information

Image
BRC No.RBRC05285
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Crtac1<tm1Kota>/Rbrc
Former Common nameCrtac1(-) (Acc. No. CDB0599K)
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeveloped by Kohtaro Takei, Yokohama City University Graduate School of Medicine.
Strain descriptionCrtac1, cartilage acidic protein 1 knockout mice. The first exon of Crtac1 was replaced with loxP-neo-loxP cassette. Homozygous mutant mice show abnormal olfactory morphology.
Colony maintenanceHeterozygote x Wild-type [C57BL/6N]
ReferencesScience, 333, 769-773 (2011). 21817055

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Crtac1cartilage acidic protein 119Crtac1<tm1Kota>targeted mutation 1, Kohtaro Takei

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP19loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)19neo; neomycin;

Ordering Information

Donor DNAE. coli neo, phage P1 loxP, mouse phosphoglycerate kinase promoter (PGK promoter), mouse PGK3’end, mouse Crtac1 genomic DNA
Research application
Specific Term and Conditions(1) The RECIPIENT shall obtain a prior agreement from the DEVELOPER (Dr. Kohtaro Takei, Yokohama City University, E-mail: kohtaro@med.yokohama-cu.ac.jp) to transfer the BIOLOGICAL RESOURCE. (2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the Acc.No. CDB0599K and Web. Site (https://large.riken.jp/distribution/mutant-list.html) is requested. (3) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designed by the DEPOSITOR is requested. (Science. 2011 333(6043):769-73.) (4) Please inform the DEPOSITOR (mutant.bdr@riken.jp) about the paper when published.
DepositorRIKEN CDB (RIKEN CDB)
Strain Statusan icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data