Strain Information | |
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Image | |
BRC No. | RBRC05286 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6.Cg-Mdga1<tm1.1Tymo>/TymoRbrc |
Former Common name | Mdga1 KO, B6-Mdga1 KO, Mdga1 (Acc. No.CDB0454K) |
H-2 Haplotype | |
ES Cell line | TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1] |
Background strain | C57BL/6JJcl |
Appearance | |
Strain development | Developed by Tohru Yamamoto, Faculty of Pharmaceutical Science, Hokkaido University. TT2 (B6CBF1 derived) ES cells were used to generate Mdga1 knockout mice. The mutant mice were backcrossed to C57BL/6J. |
Strain description | Mdga1 gene knockout mice. Exon 1 of the Mdga1 gene was replaced with a lacZ and frt flanked neo cassette (neo cassette was removed by FLPe-mediated recombination). Homozygous mutant mice exhibit defective migration and positioning of meurons during corticogenesis. |
Colony maintenance | Heterozygote x Wild-type [C57BL/6JJcl] |
References | Am. J. Med. Genet. B Neuropsychiatr. Genet., 147B, 1089-1100 (2008). 18384059 Dev. Dyn., 240, 96-107 (2011). 21104742 Brain Res., 1101, 12-19 (2006). 16782075 Schizophr. Res., 194-200 (2011). 21146959 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Mdga1MAM domain containing glycosylphosphatidylinositol anchor 117Mdga1 <tm1.1Tymo>targeted mutation 1.1, Toru Yamamoto Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site17Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter lacZbeta-galactosidase (E. coli)17 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter lox71mutant lox sequence17lox71 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP17loxP |
Ordering Information | |
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Donor DNA | E.coli LacZ, P1 phage lox71, loxP, Yeast frt, マウス PGKプロモーター,PGK 3’end, ウサギ beta-globin 3’end |
Research application | Cre/loxP system FLP/frt system Fluorescent Proteins/lacZ System |
Specific Term and Conditions | (1) The RECIPIENT shall obtain a prior agreement from the DEVELOPER (Dr. Tohru Yamamoto, Hokkaido University, E-mail: yamamoto@pharm.hokudai.ac.jp) to transfer the BIOLOGICAL RESOURCE. (2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the Acc.No. CDB0454K and Web. Site (https://large.riken.jp/distribution/mutant-list.html) is requested. (3) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designed by the DEPOSITOR is requested. (Dev Dyn. 2011 240(1):96-107.) (4) Please inform the DEPOSITOR (mutant.bdr@riken.jp) about the paper when published. |
Depositor | RIKEN CDB (RIKEN CDB) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |