Strain Data Sheet
ANIMAL SEARCH SYSTEM
Data update: Jun 20, 2019
Health Report   PCR Protocol   Thawing Method
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RBRC No. RBRC05323  
Type Targeted MutationCartagena  
Species Mus musculus  
Strain name 129-Sirt1<tm1Mcby>  
Former Common name STOCK Sirt1  
H-2 Haplotype No Data  
ES cell line R1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]  
Background strain No Data  
Appearance
 1 Appearance No Data  
Genotype No Data  
Strain development Developed by Michael McBurney, Ottawa Regional Cancer Centre and Department of Medicine, University of Ottawa. A hygromycin resistance cassette was inserted to replace exons 5 and 6. 129 background.  
Strain description SirT1 knockout mice. A hygromycin resistance cassette was inserted to replace exons 5 and 6 of Sirt1 gene. Homozygous mutant mice die at birth. When outbred to CD1, SirT1 KO mice have short snouts, eyelid defect and are smaller than littermates at weaning.  
Colony maintenance Heterozygote X Wild-type  
Health Report
Gene Details
Promoter No Data  
 1 Symbol IRES  
Symbol name internal ribosomal entry site (EMCV)  
Chromosome 10  
Common name internal ribosomal entry site from EMCV  
Symbol description No Data  
 2 Symbol Sirt1  
Symbol name sirtuin 1 (silent mating type information regulation 2, homolog) 1 (S. cerevisiae); targeted mutation 1, Michael W McBurney  
Chromosome 10  
Common name No Data  
Symbol description No Data  
Promoter herpes simplex virus thymidine kinase promoter (HSV tk promoter)  
 3 Symbol Hyg  
Symbol name hygromycin phasphotransferase (E. coli)  
Chromosome 10  
Common name No Data  
Symbol description No Data  
References   
Research applications No Data  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.  
Additional information
 1 No Data  
 2 Genotyping protocol <PCR>  
Depositor McBurney, Michael W. (Ottawa Hospital Research Institute)  McBurney, Michael W. 
Strain Status /
Availability
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