Strain Data Sheet
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Data update: Jun 20, 2019
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RBRC No. RBRC05324  
Type Targeted MutationCartagena  
Species Mus musculus  
Strain name 129-Sirt1<tm2.1Mcby>  
Former Common name Sirt1 KI/H355Y, STOCK Sirt1<tm2.1Mcby>  
H-2 Haplotype No Data  
ES cell line R1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]  
Background strain No Data  
Appearance
 1 Appearance No Data  
Genotype No Data  
Strain development Developed by Michael McBurney, Ottawa Regional Cancer Centre and Department of Medicine, University of Ottawa. A knock-in vector carrying a point mutation in exon 5 of Sirt1 gene, in which codon 355 was mutated from CAT (histidine) to TAT (tyrosine) was inserted into exon 5. A hygromycin resistant cassette flanked by loxP sites was removed by crossing with Prm-Cre mice (129-Tg (Prm-Cre) 580g / J).  
Strain description SirT1 variant (H355Y) knock-in mice, carrying a point mutation in exon 5 of Sirt1 gene, in which codon 355 was mutated from CAT (histidine) to TAT (tyrosine). Homozygous mutant mice are infertile.  
Colony maintenance Heterozygote X Wild-type  
Health Report
Gene Details
Promoter No Data  
 1 Symbol Sirt1  
Symbol name sirtuin 1 (silent mating type information regulation 2, homolog) 1 (S. cerevisiae)  
Chromosome 10  
Common name No Data  
Symbol description No Data  
 2 Symbol loxP  
Symbol name phage P1 loxP  
Chromosome 10  
Common name No Data  
Symbol description No Data  
References   
Research applications Cre/loxP system  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.  
Additional information
 1 No Data  
 2 Genotyping protocol <PCR>  
Depositor McBurney, Michael W. (Ottawa Hospital Research Institute)  McBurney, Michael W. 
Strain Status /
Availability
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