Strain Data Sheet
ANIMAL SEARCH SYSTEM
Data update: May 20, 2019
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RBRC No. RBRC05382  
Type TransgeneCartagena  
Species Mus musculus  
Strain name B6.Cg-Tg(Dppa3-ECFP)#Sait/Rbrc  
Former Common name Stella-ECFP (Acc. No. CDB0465T)  
H-2 Haplotype No Data  
Background strain No Data  
Appearance
 1 Appearance No Data  
Genotype No Data  
Strain development Developed by Yasuhide Ohinata and Mitinori Saitou, RIKEN, Center for Developmental Biology in 2008. Transgene was injected into the pronuclei of B6D2F2 fertilized eggs. The mice were crossed to C57BL/6.  
Strain description The transgenic mice having a Dppa3-ECFP containing upstream sequence, exon 1 through exon 4 of Dppa3 with a ECFP reporter sequence that inserted in-frame after the initial ATG of exon 2 of the Dppa3. Tg homozygous females have low fertility.  
Colony maintenance Carrier x Nocarrier [C57BL/6N]  
Health Report
Gene Details
Promoter mouse Dppa3 promoter  
 1 Symbol ECFP  
Symbol name Enhanced Cyan Fluorescent Protein (Jellyfish)  
Chromosome 1  
Common name No Data  
Symbol description No Data  
References Reproduction. 2008 Oct;136(4):503-14. Epub 2008 Jun 26.
  
A comprehensive, non-invasive visualization of primordial germ cell development in mice by the Prdm1-mVenus and Dppa3-ECFP double transgenic reporter.
A comprehensive, non-invasive visualization of primordial germ cell development in mice by the Prdm1-mVenus and Dppa3-ECFP double transgenic reporter.
Research applications Fluorescent Proteins/lacZ System  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
(1) The RECIPIENT shall obtain a prior agreement from the DEVELOPER (Dr. Mitinori Saitou, Kyoto University, E-mail: saitou@anat2.med.kyoto-u.ac.jp) to transfer the BIOLOGICAL RESOURCE. (2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the Acc.No. CDB0465T and Web. Site (http://www.cdb.riken.jp/arg/mutant%20mice%20list.html) is requested. (3) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designed by the DEPOSITOR is requested. (Reproduction. 2008 136(4):503-14.) (4) Please inform the DEPOSITOR (mutant@cdb.riken.jp) about the paper when published.  
Additional information
 1 Mouse of the Month Aug 2013  
 2 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.  
 3 Genotyping protocol <PCR>  
Depositor RIKEN, CDB (RIKEN Center for Developmental Biology)  RIKEN, CDB 
Strain Status /
Availability
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