Strain Data Sheet
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Data update: Jun 20, 2019
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RBRC No. RBRC05447  
Type TransgeneCartagena  
Species Mus musculus  
Strain name B6.Cg-Tg(ACTB-tTS)1Mllo  
Former Common name Actin-tTS Tg, B6-ACTB-tTS Tg  
H-2 Haplotype No Data  
Background strain No Data  
Appearance
 1 Appearance No Data  
Genotype No Data  
Strain development Developed by Moises Mallo, Max-Plank Institute of Immunobiology. C57BL/6 and 129 mixed background.  
Strain description ACTB-tTS transgenic mice. These mice express tetracycline-controlled transcriptional silencer (tTS) gene under the control of the human beta actin promoter. By the combination with homozygous tetO-modified allele, expression from the gene can be blocked in the absence of doxycycline and restore when the antibiotic is administered to the animal.  
Colony maintenance Carrier x Noncarrier [C57BL/6JJcl]  
Health Report
Gene Details
Promoter No Data  
 1 Symbol No Data  
Symbol name SV40 polyA signal  
Chromosome UN  
Common name No Data  
Symbol description No Data  
Promoter human beta-actin promoter, E. coli Tetracycline repressor (tetR), human Zfp KRAB domain  
 2 Symbol tTS  
Symbol name tTS (E. coli Tetracycline repressor (tetR), human ZFP KRAB domain cDNA)  
Chromosome UN  
Common name No Data  
Symbol description No Data  
References Genomics. 2003 Apr;81(4):356-60.
  
Reversible gene inactivation in the mouse.
Reversible gene inactivation in the mouse.
Research applications Tet system  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEVELOPER (Dr. Moises Mallo, (Max-Plank Institute of Immunobiology) is requested. Genomics, 81(4):356-360 2003. The RECIPIENT must contact the DEVELOPER in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEVELOPER and must obtain a prior written consent from the DEVELOPER.  
Additional information
 1 No Data  
 2 Genotyping protocol <PCR>  
Depositor Tanaka, Kenji (Keio University)  Tanaka, Kenji 
Strain Status /
Availability
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