Strain Information | |
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Image | |
BRC No. | RBRC05451 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6;129-Nlgn3<tm1(STOP-tetO)Rhn> |
Former Common name | Nlgn3 STOPtetO KI, B6-Nlgn3/STOPtetO KI |
H-2 Haplotype | |
ES Cell line | |
Background strain | |
Appearance | |
Strain development | Developed by Kenji Tanaka, Columbia University in 2007. CSL3 ES cells derived from 129S6/SvEvTac were used. C57BL/6 and 129 mixed background. |
Strain description | Nlgn3 knockout mice. Neo-STOP-tetO (loxP-FRT-Neo-STOP-FRT-tetO-loxP) cassette was inserted just upstream of the Nlgn3 gene. Homozygous mutant mice show no obvious abnormality. tet0 mediated allele can be generated by crossing with FLP mice. |
Colony maintenance | Homozygote x Hemizygote [or Crossing to C57BL/6JJcl] |
References | Biol. Psychiatry, 67, 770-773 (2010). 20163789 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) siteXFrt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) siteXFrt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) siteXFrt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxPXloxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxPXloxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)Xneo; neomycin;mouse Phosphoglycerate kinase promoter (PGK promoter), synthetic bacterial EM7 promoter (T7 phage) Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter tetOtetracycline operator (E. coli)XtetO |
Ordering Information | |
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Donor DNA | P1 Phage loxP sites, yeast FRT (flipase recombination target) sites, mouse PGK promoter, synthetic bacterial EM7 promoter (T7 phage), E. coli neo, yeast His3 3' UTR, SV40 virus SV40 polyA signal, E. coli Tetracycline operator site, Cytomegalovirus CMV minimum promoter, mouse Nlgn3 genomic DNA |
Research application | Cre/loxP system FLP/frt system Tet system |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Biol. Psychiatry, 67, 770-773 (2010). |
Depositor | Kenji Tanaka (Keio University) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |