Strain Data Sheet
ANIMAL SEARCH SYSTEM
Data update: Aug 20, 2019
Health Report   PCR Protocol   Thawing Method
embryos / sperm >
  Mouse Marking
Figure
  Order
RBRC No. RBRC05453  
Type TransgeneCartagena  
Species Mus musculus  
Strain name B6(Cg)-Tg(tetO-ChR2*C128S/EYFP)1Kftnk  
Former Common name tetO-ChR2 BAC Tg, B6-tetO-ChR2/YFP Tg  
H-2 Haplotype No Data  
Background strain No Data  
Appearance
 1 Appearance No Data  
Genotype No Data  
Strain development Developed by Kenji Tanaka and Akihiro Yamanaka, National Institute for Physiological Sciences. The transgene was injected into the pronuclei of C57BL/6J fertilized eggs. FRT-flanked neo selection cassette was removed by crossing with ROSA-Flpe mice. C57BL/6 and 129 mixed background.  
Strain description tetO-ChR2 (C128S) BAC transgenic mice. Linearized modified BAC DNA containing the Actb gene followed by a tet0-ChR2 cassette was injected into C57BL/6 fertilized eggs.  
Colony maintenance Carrier x Noncarrier [C57BL/6JJcl]  
Health Report
Gene Details
Promoter No Data  
 1 Symbol No Data  
Symbol name SV40 polyA signal  
Chromosome UN  
Common name No Data  
Symbol description No Data  
 2 Symbol EYFP  
Symbol name Enhanced Yellow Fluorescent Protein (Jellyfish)  
Chromosome UN  
Common name No Data  
Symbol description No Data  
 3 Symbol Frt  
Symbol name yeast FRT (flippase recombination target) site  
Chromosome UN  
Common name No Data  
Symbol description No Data  
 4 Symbol loxP  
Symbol name phage P1 loxP  
Chromosome UN  
Common name No Data  
Symbol description No Data  
Promoter E. coli Tetracycline operator, CMV minimal promotor, rabbit Beta globin intron  
 5 Symbol ChR2  
Symbol name channelrhodopsin-2 (Chlamydomonas)  
Chromosome UN  
Common name No Data  
Symbol description No Data  
References   
Research applications Cre/loxP system,
FLP/frt system,
Fluorescent Proteins/lacZ System,
Tet system  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Cell Rep., 2, 397-406 (2012).
The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. Prior to requesting the BIOLOGICAL RESOURCE, RECIPIENT must inform the DEPOSITOR of his project using the Approval Form. If the
use of this BIOLOGICAL RESOURCE results in scientific publications, the RECIPIENT should cite Kenji F. Tanaka as co-author in the first paper.  
Additional information
 1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.  
 2 Genotyping protocol <PCR>  
Depositor Tanaka, Kenji (Keio University)  Tanaka, Kenji 
Strain Status /
Availability
(Expected delivery)

back Back
RIKEN BioResource Research Center

English page click here ANIMAL SEARCH SYSTEM