Strain Data Sheet
ANIMAL SEARCH SYSTEM
Data update: Jun 20, 2019
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RBRC No. RBRC05457  
Type Targeted MutationCartagena  
Species Mus musculus  
Strain name B6.B6CB-Nedd9<tm1Miku>  
Former Common name Cas-L KO B6-Nedd9 KO  
H-2 Haplotype No Data  
ES cell line TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]  
Background strain No Data  
Appearance
 1 Appearance No Data  
Genotype No Data  
Strain development Developed by Sachiko Seo, Faculty of Medicine, University of Tokyo in 2001. The targeting vector was transferred into TT2 ES cells derived from (C57BL/6 x CBA) F1. The mutant mice were backcrossed to C57BL/6.  
Strain description Cas-L (Nedd9, neural precursor cell expressed, developmentally down-regulated gene 9) gene knockout mice. A 1.2 kb region including exon 2 was replaced with EGFP and a floxed neomycin resistant cassette. Homozygous mutant mice show reduced numbers of lymphocytes in secondary lymphoid organs and an almost complete loss of marginal zone B (MZB) cells in the spleen.  
Colony maintenance Heterozygote x Wild-type [C57BL/6JJcl]  
Health Report No Data  
Gene Details
Promoter No Data  
 1 Symbol Nedd9  
Symbol name neural precursor cell expressed, developmentally down-regulated gene 9  
Chromosome 13  
Common name No Data  
Symbol description No Data  
 2 Symbol EGFP  
Symbol name Enhanced Green Fluorescent Protein, F64L and S65T (Jellyfish)  
Chromosome 13  
Common name No Data  
Symbol description No Data  
 3 Symbol loxP  
Symbol name phage P1 loxP  
Chromosome 13  
Common name No Data  
Symbol description No Data  
Promoter herpes simplex virus thymidine kinase promoter (HSV tk promoter)  
 4 Symbol neo  
Symbol name neomycin resistance gene (E. coli)  
Chromosome 13  
Common name neo; neomycin;  
Symbol description No Data  
References

  


Research applications Cre/loxP system,
Fluorescent Proteins/lacZ System  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
J. Immunol., 175, 3492-3501 (2005).  
Additional information
 1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.  
 2 Genotyping protocol <PCR>  
Depositor Kurokawa, Mineo (The University of Tokyo)  Kurokawa, Mineo 
Strain Status /
Availability
(Expected delivery)

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