Strain Data Sheet
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Data update: Jun 20, 2019
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RBRC No. RBRC05467  
Type Targeted MutationCartagena  
Species Mus musculus  
Strain name B6.Cg-Gt(ROSA)26Sor<tm4.1(CAG-mCitrine)Maoh>  
Former Common name B6-ROSA26/CAG-mCitrine  
H-2 Haplotype No Data  
ES cell line E14TG2a [129P2/OlaHsd]carries Hprt  
Background strain No Data  
Appearance
 1 Appearance No Data  
Genotype No Data  
Strain development Develop by Masato Ohtsuka, Tokai University School of Medicine in 2010. The mice were backcrossed to C57BL/6JJcl. Extra sequence in the RMCE<ex> was removed by FLP-mediated recombination.  
Strain description The mice having a CAG-mCitrine-polyA cassette at the ROSA26 locus that were generated by RMCE (Recombinase-mediated cassette exchange). The mutant mice exhibit ubiquitous and very bright fluorescence.  
Colony maintenance Homozygote x Homozygote [or Crossing to C57BL/6JJcl]  
Health Report
Gene Details
Promoter No Data  
 1 Symbol Gt(ROSA)26Sor  
Symbol name gene trap ROSA 26, Philippe Soriano  
Chromosome 6  
Common name No Data  
Symbol description No Data  
 2 Symbol No Data  
Symbol name SV40 polyA  
Chromosome 6  
Common name No Data  
Symbol description No Data  
 3 Symbol Frt  
Symbol name yeast FRT (flippase recombination target) site  
Chromosome 6  
Common name No Data  
Symbol description No Data  
 4 Symbol lox2272  
Symbol name mutant lox sequence  
Chromosome 6  
Common name No Data  
Symbol description No Data  
Promoter CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)  
 5 Symbol mCitrine  
Symbol name variant of Yellow Fluorescent Protein  
Chromosome 6  
Common name No Data  
Symbol description No Data  
References Nucleic Acids Res. 2010 Dec 1;38(22):e198. Epub 2010 Sep 29.  
Pronuclear injection-based mouse targeted transgenesis for reproducible and highly efficient transgene expression.
Research applications Cell Biology Research,
Cre/loxP system,
FLP/frt system,
Fluorescent Proteins/lacZ System  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nucleic Acids Res., 38, e198 (2010). For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.  
Additional information
 1 No Data  
 2 Genotyping protocol <PCR>  
Depositor Ohtsuka, Masato (Tokai University)  Ohtsuka, Masato 
Strain Status /
Availability
(Expected delivery)

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