Strain Data Sheet

RBRC05467

Strain Information

Image
BRC No.RBRC05467
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Gt(ROSA)26Sor<tm4.1(CAG-mCitrine)Maoh>
Former Common nameB6-ROSA26/CAG-mCitrine
H-2 Haplotype
ES Cell lineE14TG2a [129P2/OlaHsd]
Background strain
Appearance
Strain developmentDevelop by Masato Ohtsuka, Tokai University School of Medicine in 2010. The mice were backcrossed to C57BL/6JJcl. Extra sequence in the RMCE<ex> was removed by FLP-mediated recombination.
Strain descriptionThe mice having a CAG-mCitrine-polyA cassette at the ROSA26 locus that were generated by RMCE (Recombinase-mediated cassette exchange). The mutant mice exhibit ubiquitous and very bright fluorescence.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6JJcl]
ReferencesNucleic Acids Res., 38, e198 (2010). 20880997

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Gt(ROSA)26Sorgene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sor<tm4.1(CAG-mCitrine)Maoh>targeted mutation 4.1, Masato Ohtsuka

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site6Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lox2272phage P1 loxP variant6lox2272

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
mCitrineYellow Fluorescent Protein (Aequorea victoria)6mCitrineCAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 polyA6

Ordering Information

Donor DNAPhage P1 lox2272 site, CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), jellyfish mCitrine cDNA, SV40 polyA, yeast FRT site, mouse Gt(ROSA)26Sor genomic DNA
Research applicationCell Biology Research
Cre/loxP system
FLP/frt system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nucleic Acids Res., 38, e198 (2010). For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorMasato Ohtsuka (Tokai University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. CAGGS MTA (English)
  4. UCSD MTA
  5. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data