Strain Information | |
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Image | |
BRC No. | RBRC05470 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | C.Cg-Gt(ROSA)26Sor<tm3.1(CAG-mCFP)Maoh> |
Former Common name | BALB-ROSA26/CAG-mCFP |
H-2 Haplotype | |
ES Cell line | E14TG2a [129P2/OlaHsd] |
Background strain | |
Appearance | |
Strain development | Develop by Masato Ohtsuka, Tokai University School of Medicine in 2010. The mice were backcrossed to BALB/cAJcl. Extra sequence in the RMCE<ex> was removed by FLP-mediated recombination. |
Strain description | The mice having a CAG-mCFP-polyA cassette at the ROSA26 locus that were generated by RMCE (Recombinase-mediated cassette exchange). The mutant mice exhibit ubiquitous and very bright fluorescence. |
Colony maintenance | Homozygote x Homozygote [or Crossing to BALB/cAJcl] |
References | Nucleic Acids Res., 38, e198 (2010). 20880997 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gt(ROSA)26Sorgene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sor<tm3.1(CAG-mCFP)Maoh>targeted mutation 3.1, Masato Ohtsuka |
Ordering Information | |
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Donor DNA | CAGプロモーター, mCFP, SV40 polyA, FRT, lox2272 |
Research application | Cre/loxP system FLP/frt system Fluorescent Proteins/lacZ System |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nucleic Acids Res., 38, e198 (2010).For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR. |
Depositor | Masato Ohtsuka (Tokai University) |
Strain Status | Frozen sperm |
Strain Availability | Recovery and QC required prior to distribution |
Additional Info. | Necessary documents for ordering: |
BRC mice in Publications |
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No Data |