Strain Information | |
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Image | |
BRC No. | RBRC05477 |
Type | Transgene |
Species | Mus musculus |
Strain name | B6.Cg-Tg(NDE-hsp-Cluap1,lacZ)41Hmd |
Former Common name | NDE-hsp Qilin-lacZ (41), B6-NDE-Qilin/lacZ Tg#41 |
H-2 Haplotype | |
ES Cell line | |
Background strain | |
Appearance | |
Strain development | Developed by Hiroshi Hamada, Graduate School of Frontier Biosciences, Osaka University. 0.7 kb segment containing Nodal NDE enhancer and Qilin cDNA-IRES LacZ genes were injected into the pronuclei of ((C57BL/6 x BALB/c)F1 x B6129-Qilin<tm1.1Hmd>)F1 fertilized eggs (2 copies). C57BL/6 and 129 mixed background. |
Strain description | This transgenic mouse expresses Cluap1 (Qilin) and lacZ genes in crown cells of embryonic node. Tg expression can be monitored by X-gal staining. |
Colony maintenance | Carrier x Noncarrier [C57BL/6JJcl] |
References | Dev. Biol., 381(1), 203-212 (2013). 23742838 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Cluap1clusterin associated protein 1UNCluap1Qilinmouse Nodal NDE-enhancer, mouse Hsp68 promoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter lacZbeta-galactosidase (E. coli)UN Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxPUNloxP |
Ordering Information | |
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Donor DNA | Mouse Nodal NDE-enhancer, Mouse Hsp68 promoter, Mouse Qilin gene cDNA, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), E. coli LacZ gene, phage P1 loxP sequence |
Research application | Cre/loxP system Developmental Biology Research Fluorescent Proteins/lacZ System |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Dev. Biol., 381(1), 203-212 (2013). |
Depositor | Hiroshi Hamada (RIKEN CDB) |
Strain Status | Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |