Strain Information | |
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Image | |
BRC No. | RBRC05496 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6;129P2-Alk<tm1(hALK*R1275Q)Homy> |
Former Common name | ALK KI |
H-2 Haplotype | |
ES Cell line | |
Background strain | |
Appearance | |
Strain development | Developed by Hiroaki Honda, Research Institute for Radiation Biology and Medicine, Hiroshima University. E14 ES cells (129P2/OlaHsd) were used. The mutant mice were crossed to C57BL/6N mice. |
Strain description | Human ALK (R1275Q variant) gene knock-in mice. hALK (R1275Q) cDNA-IRES-beta geo-polyA was inserted into the middle of the exon 21 and exon 22. |
Colony maintenance | Heterozygote x Wild-type [C57BL/6NCrlCrlj] |
References |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter ALKanaplastic lymphoma kinase (human)17ALK Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter IRESinternal ribosomal entry site (EMCV)17internal ribosomal entry site from EMCV Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter lacZbeta-galactosidase (E. coli)17 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP17loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter poly ApolyA17poly A |
Ordering Information | |
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Donor DNA | mouse ALK genomic DNA, human ALK cDNA, Phage P1 LoxP, E. coli beta-galactosidase, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), polyA sequence (synthetic DNA) |
Research application | |
Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. |
Depositor | Hiroaki Honda (Tokyo Women's Medical University) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |