Strain Data Sheet
RBRC05525
Strain Information | |
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| Image | |
| BRC No. | RBRC05525 |
| Type | Transgene |
| Species | Mus musculus |
| Strain name | B6.B6D2-Tg(Prrx1-Sox9,-lacZ)1Haak |
| Former Common name | Prx1-Sox9 Tg, Prx1-Sox9 transgenic, B6-Prx1-Sox9/lacZ Tg |
| H-2 Haplotype | |
| ES Cell line | |
| Background strain | |
| Appearance | |
| Strain development | Developed by Haruhiko Akiyama, The University of Texas MD Anderson Cancer Center. The transgene was injected into the pronuclei of (C57BL/6 x DBA/2)F1 fertilized eggs. The mutant mice were crossed to C57BL/6. |
| Strain description | Prx1-Sox9/lacZ transgenic mice in which Sox9 is specifically expressed in limb bud mesenchyme. Prx1-Sox9/lacZ transgene contains a 2.4 kb of the Prx1 limb specific promoter encoding the 3xHA-epitope tagged Sox9 cDNA followe by an IRES lacZ. Tg mice exhibit pre- and postaxial extra-digit formation. lacZ expression was detected in the limb buds from E9.5 |
| Colony maintenance | Carrier x Noncarrier [C57BL/6NCrSlc] |
| References | Matrix Biol., 26, 224-233 (2007). 17222543 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |
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| Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter GHGrowth hormone polyA (Bovine)UNGH Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter HAhuman influenza virus HA (hemagglutinin) tag sequenceUNHA Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter IRESinternal ribosomal entry site (EMCV)UN Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SOX9SRY-box containing gene 9 (human)UNSOX9mouse Prx1 promoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter lacZbeta-galactosidase (E. coli)UN |
Ordering Information | |
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| Donor DNA | mouse Prx1 promoter, human influenza virus HA (hemagglutinin) tag sequence, human SOX9 cDNA, EMCV internal ribosomal entry site (ires), E. coli lacZ, bovine polyA signal |
| Research application | Developmental Biology Research Fluorescent Proteins/lacZ System |
| Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Matrix Biol., 26, 224-233 (2007).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and Dr. Haruhiko Akiyama, Kyoto University, is requested. Depositor retains ownership of the Biological Resource, including any Biological Resource contained in any modification. Any Biological Resource delivered pursuant to the Agreement is understood to be experimental in nature and may have hazardous properties. The DEPOSITOR MAKES NO REPRESENTATIONS AND EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED. THERE ARE NO EXPRESS OR IMPLIED WARRANTIES OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR THAT THE USE OF THE BIOLOGICAL RESOURCE WILL NOT INFRINGE ANY PATENT, COPYRIGHT, TRADEMARK, OR OTHER PROPRIETARY RIGHTS. In no event shall Depositor be liable for any use by recipient of the Biological Resource for any loss, claim, damage or liability, of whatsoever kind of nature, which may arise from or in connection with the use, handling or storage of the Biological Resource. Except where limited by Federal law, or by the constitution and laws of the state governing the recipient, the recipient agrees to hold harmless Depositor and The University of Texas System and their Regents, officers, agents and employees, from any liability, loss or damage they may suffer as a result of claims, demands, costs or judgments against them arising out of recipient’s use of the Material and the use by recipient of the results obtained from the Research. The Biological Resource and modifications containing the Biological Resource may only be transferred to non-profit institutions for academic research purposes. |
| Depositor | Benoit de Crombrugghe (The University of Texas M. D. Anderson Cancer Center) |
| Strain Status |  Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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| No Data |