Strain Data Sheet

RBRC05538

Strain Information

Image
BRC No.RBRC05538
TypeGene TrapCartagena
SpeciesMus musculus
Strain nameB6;129-Orc4<Gt(ROSABetageo)CN9Yiw>
Former Common nameB6-Orc4 Gt#CN9
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strain
Appearance
Strain developmentDeveloped by Naruse Chie and Yoichiro Iwakura, The Institute of Medical Science, The University of Tokyo in 2001. C57BL/6J and 129 mixed background.
Strain descriptionOrc4 gene trapped mice. Homozygous mutant mice are embryonic lethal.
Colony maintenanceHeterozygote x Wild-type [or Crossing to C57BL/6JJmsSlc]
References

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Orc4origin recognition complex, subunit 42Orc4<Gt(ROSABetageo)CN9Yiw>gene trap CN9, Yoichiro Iwakura

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lacZbeta-galactosidase (E. coli)2

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)2neo; neomycin;

Ordering Information

Donor DNANeomycin resistance gene, lacZ gene (E. coli), TK promoter (HSV), LTR (Mo-MuLV)
Research application
Specific Term and ConditionsPlease obtain the written consent from Professor Yoichiro Iwakura (iwakura@rs.tus.ac.jp; Research Institute for Biological Sciences, Tokyo University of Science) for the use. Please inform Professor Masahide Asano (asano@kiea.m.kanazawa-u.ac.jp; Division of Transgenic Animal Science, Kanazawa University Advanced Science Research Center) about the use.
DepositorYoichiro Iwakura (The University of Tokyo)
Strain Statusan icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data