Strain Data Sheet
Data update: Jun 20, 2019
Health Report   PCR Protocol   Thawing Method
embryos / sperm >
  Mouse Marking
RBRC No. RBRC05541  
Type Targeted MutationCartagena  
Species Mus musculus  
Strain name B6.129-Zfp277<tm1>  
Former Common name Zfp277 KO, B6-Zfp277 KO  
H-2 Haplotype No Data  
ES cell line R1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]  
Background strain No Data  
 1 Appearance No Data  
Genotype No Data  
Strain development Developed by Atsushi Iwama, Graduate School of Medicine, Chiba University in 2005. The mutant mice were backcrossed to C57BL/6N.  
Strain description Zfp277 gene knockout mice. Exon 5 of Zfp277 gene was replaced with a floxed neo cassette. Homozygous mutant mice were born according to Menderian's law, but the growth retardation and death of homozygous mice were sporadically observed.  
Colony maintenance Heterozygote x Wild-type [C57BL/6NCrSlc]  
Health Report
Gene Details
Promoter No Data  
 1 Symbol Zfp277  
Symbol name zinc finger protein 277  
Chromosome 12  
Common name No Data  
Symbol description No Data  
 2 Symbol loxP  
Symbol name phage P1 loxP  
Chromosome 12  
Common name No Data  
Symbol description No Data  
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)  
 3 Symbol neo  
Symbol name neomycin resistance gene (E. coli)  
Chromosome 12  
Common name neo; neomycin;  
Symbol description No Data  
Research applications Cre/loxP system  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
PloS One., 5, e12373 (2010).  
Additional information
 1 No Data  
 2 Genotyping protocol <PCR>  
Depositor Iwama, Atsushi (Graduate School of Medical and Pharmaceutical Sciences, Chiba University)  Iwama, Atsushi 
Strain Status /
(Expected delivery)

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