Strain Data Sheet

RBRC05550

Strain Information

Image
BRC No.RBRC05550
TypeTargeted Mutation and TransgeneCartagena
SpeciesMus musculus
Strain nameB6.Cg-Ighm<tm1Cgn> Tg(Aicda-cre)9Mbu Gt(ROSA)26Sor<tm1Sho>
Former Common namemuMT/Aicda-cre/R26R, B6-Ighm KO/AicdaCre/ROSA26-lacZ
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentmuMT : Klaus Rajewsky, Insitute for Genetics, University of Cologne. R26R : Philippe Soriano, Mount Sinal School of Medicine. Aicda-cre : Meinrad Busslinger. Research Institute of Molecular Pathology.
Strain descriptionThis strain has Ighm knockout (Ighm<tm1Cgn>), AID-Tg (Tg(Aicda-cre)9Mbu) and ROSA26 lacZ reporter (Gt(ROSA)26Sor<tm1Sho>) alleles. Immunodeficient.
Colony maintenanceIghm : Homozygote x Homozygote ; Tg : Carrier x Carrier ; ROSA26 : Homozygote x Homozygote
ReferencesImmunity, 28, 751-762 (2008). 18538592
PLoS One, 6, e29141 (2011). 22216188
Nature, 350, 423-436 (1991). 1901381
Proc. Natl. Acad. Sci. USA, 96, 5037-5042 (1999). 10220414

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Ighmimmunoglobulin heavy constant mu12Ighm<tm1Cgn>targeted mutation 1, University of Cologne

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Gt(ROSA)26Sorgene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sor<tm1Sho>targeted mutation 1, Stuart Orkin

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)6neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
CD2CD2 (human)UNCD2

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Iresinternal ribosomal entry site (EMCV)UN

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
crePhage P1 Cre recombinaseUNcremouse Aicda promoter

Ordering Information

Donor DNAherpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neomycin phosphotransferase, polyA, mouse Ighm genomic DNA, mouse Aicda genomic DNA (BAC clone RP24-68I7), SV40 nuclear localization sequence, Phage P1 Cre recombinase, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), human CD2 cDNA, SV40 polyA signal, retrovirus LTR, adenovirus major late transcript splice acceptor (SA) sequence, Phage P1 loxP sites, E.coli lacZ, E. coli neomycin phosphotransferase, bovine growth hormone polyA, mouse ROSA26 locus genomic DNA
Research applicationCre/loxP system
Specific Term and Conditions1) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested. PLoS One. 2011 6(12):e29141.
2) The RECIPIENT shall use the BIOLOGICAL RESOURCE only for academic research for the purpose of publishing the research results.
3) The BIOLOGICAL RESOURCE contains Tg(Aicda-re)9Mbu and Gt(ROSA)26Sortm1Sho. Before executing the MTA between RIKEN BRC and the RECIPIENT, the RECIPIENT shall contact Dr. Meinrad Busslinger, Research Institute of Molecular Pathology and shall execute the material transfer agreement with Research Institute of Molecular Pathology to use Tg(Aicda-cre)9Mbu. The RECIPIENT shall obtain the prior written consent of Dr. Philippe Soriano, Mount Sinai School of Medicine to use Gt(ROSA)26Sortm1Sho.
DepositorTasuku Honjo (Kyoto University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-
Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)
Honjo Lab HP

BRC mice in Publications

No Data