Strain Data Sheet

RBRC05558

Strain Information

Image
BRC No.RBRC05558
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain name129(Cg)-Rax<tm1.1Tfur>
Former Common name129-Rax floxed
H-2 Haplotype
ES Cell lineTC1 [129S6/SvEvTac]
Background strain129S6/SvEvTac
Appearance
Strain developmentDeveloped by Takahisa Furukawa, Osaka Bioscience Institute in 2006. 129 background. A Frt-flanked PGK-neo selection cassette was removed by crossing with FLP1 mice (B6;SJL-Tg(ACTFLPe)9205Dym/J).
Strain descriptionRax gene floxed mice. Exons 1 and 2 of the Rax gene were flanked by loxP sites. Conditional Rax deficient mice can be generated by crossing with tissue-specific Cre mice.
Colony maintenanceHomozygote x Homozygote [or Crossing to 126S6/SvEvTac]
ReferencesJ. Neurosci., 31, 16792-16807 (2011). 22090505

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Raxretina and anterior neural fold homeobox18Rax<tm1.1Tfur>targeted mutation 1.1, Takahisa Furukawa

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site18Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP18loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP18loxP

Ordering Information

Donor DNABacteriophage P1 loxP sites, Saccharomyces cerevisiae frt site, mouse Rax genomic DNA
Research applicationCre/loxP system
FLP/frt system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Neurosci., 31, 16792-16807 (2011).Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must contract an MTA with the DEPOSITOR (Osaka University).
DepositorTakahisa Furukawa (Osaka Bioscience Institute)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data