Strain Data Sheet
ANIMAL SEARCH SYSTEM
Data update: Jun 20, 2019
Health Report   PCR Protocol   Thawing Method
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RBRC No. RBRC05601  
Type Targeted MutationCartagena  
Species Mus musculus  
Strain name B6.129(Cg)-Rab7<tm1.2Ywd>  
Former Common name Rab7 null, B6-rAB7 KO  
H-2 Haplotype No Data  
ES cell line R1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]  
Background strain No Data  
Appearance
 1 Appearance No Data  
Genotype No Data  
Strain development Developed by Yoh Wada, Institute of Scientific and Industrial Research, Osaka University. An Rab7 targeted allele, containing a loxP site in upstream of exon 2 and a RT-loxP-Neo-FRT-loxP cassette in downstream of exon 13, was introduced into the mouse genome by R1 ES cell mediated gene replacement. The modified allele was converted to Rab7- (Rab7<tm1.1Ywd>) by crossing with FLP and Cre mice (B6;SJL-Tg(ACTFLPe)9205Dym/J and FVB/N-Tg(EIIa-cre)C5379Lmgd/J). The mutant mice were cross to C57BL/6.  
Strain description Rab7 gene knockout mice. Exons 2 and 3 of the Rab7 gene were removed. Homozygous mutant mice are embryonic lethal.  
Colony maintenance Heterozygote x Wild-type [C57BL/6JJmsSlc]  
Health Report
Gene Details
Promoter No Data  
 1 Symbol Frt  
Symbol name yeast FRT (flippase recombination target) site  
Chromosome 6  
Common name No Data  
Symbol description No Data  
 2 Symbol Rab7  
Symbol name RAB7, member RAS oncogene family  
Chromosome 6  
Common name No Data  
Symbol description No Data  
 3 Symbol loxP  
Symbol name phage P1 loxP  
Chromosome 6  
Common name No Data  
Symbol description No Data  
References   
Research applications Cell Biology Research,
Cre/loxP system,
FLP/frt system  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers. The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR. RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or research in non-profit organization is requested to enter into a Material Transfer Agreement with Osaka University.  
Additional information
 1 No Data  
 2 Genotyping protocol <PCR>  
Depositor Wada, Yoh (The institute of scientific and industrial research, osaka university)  Wada, Yoh 
Strain Status /
Availability
(Expected delivery)

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