Strain Information | |
---|---|
Image | |
BRC No. | RBRC05601 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6.129(Cg)-Rab7<tm1.2Ywd> |
Former Common name | Rab7 null, B6-rAB7 KO |
H-2 Haplotype | |
ES Cell line | R1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>] |
Background strain | |
Appearance | |
Strain development | Developed by Yoh Wada, Institute of Scientific and Industrial Research, Osaka University. An Rab7 targeted allele, containing a loxP site in upstream of exon 2 and a RT-loxP-Neo-FRT-loxP cassette in downstream of exon 13, was introduced into the mouse genome by R1 ES cell mediated gene replacement. The modified allele was converted to Rab7- (Rab7<tm1.1Ywd>) by crossing with FLP and Cre mice (B6;SJL-Tg(ACTFLPe)9205Dym/J and FVB/N-Tg(EIIa-cre)C5379Lmgd/J). The mutant mice were cross to C57BL/6. |
Strain description | Rab7 gene knockout mice. Exons 2 and 3 of the Rab7 gene were removed. Homozygous mutant mice are embryonic lethal. |
Colony maintenance | Heterozygote x Wild-type [C57BL/6JJmsSlc] |
References | Cell Rep., 31(10):107733 (2022). 32521258 Nat. Commun., 3, 1071 (2012). 22990867 Dev. Cell, 22, 1163-1175 (2012). 22698281 |
Health Report | |
---|---|
Examination Date / Room / Rack |
Gene | |
---|---|
Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site6Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP6loxP |
Ordering Information | |
---|---|
Donor DNA | Escherichia coli Tn7 Right border and left border, Bacteriophage P1 LoxP site, Saccharomyces cerevisiae Frt site, Escherichia coli Tn5 Outer elements, mouse Rab7 genomic DNA |
Research application | Cell Biology Research Cre/loxP system FLP/frt system |
Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR. RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or research in non-profit organization is requested to enter into a Material Transfer Agreement with Osaka University. |
Depositor | Yoh Wada (Osaka University) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
---|
No Data |