Strain Data Sheet
RBRC05646
Strain Information | |
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| Image | |
| BRC No. | RBRC05646 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | B6.Cg-Plch2<tm1Kfu>/KfuRbrc |
| Former Common name | B6-Plch2 KO, PLCeta(Acc.No.CDB0463K) |
| H-2 Haplotype | |
| ES Cell line | TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1] |
| Background strain | |
| Appearance | |
| Strain development | Developed by Laboratory for Animal Resources and Genetic Engineering, RIKEN Center for Developmental Biology and Fukami Kiyoko, Tokyo University of Pharmacy and Life Sciences. |
| Strain description | PLCeta2 (Plch2) gene knockout mice. The first to third exons of PLCeta2 gene were replaced with a loxP/LacZ/frt-flanked neo/loxP/pA cassette. Homozygous mutant mice show no obvious abnormality. |
| Colony maintenance | Heterozygote x Wild-type [C57BL/6N] |
| References | Gene Expr. Patterns, 10, 119-126 (2010). 20060933 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |
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| Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site4Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site4Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter lacZbeta-galactosidase (E. coli)4 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter lox71mutant lox sequence4lox71 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP4loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)4neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter) |
Ordering Information | |
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| Donor DNA | Phage P1 lox71 site, E. coli lacZ, rabbit beta globin genomic DNA, yeast FRT (flippase recombination target) sites, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neomycin resistance gene, Phage P1 loxP site, mouse Plch2 genomic DNA |
| Research application | Cre/loxP system FLP/frt system Fluorescent Proteins/lacZ System |
| Specific Term and Conditions | (1) The RECIPIENT shall obtain a prior agreement from the Dr.Kiyoko Fukami (E-mail: kfukami@toyaku.ac.jp) to transfer the BIOLOGICAL RESOURCE. (2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the Acc.No. CDB0463K and Web. Site (https://large.riken.jp/distribution/mutant-list.html) is requested. (3) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designed by the DEPOSITOR is requested. Gene Expr Patterns. 2010 10(2-3):119-26. (4) Please inform the DEPOSITOR (mutant.bdr@riken.jp) about the paper when published. |
| Depositor | RIKEN CDB (RIKEN CDB) |
| Strain Status |  Frozen embryos Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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| No Data |