Strain Data Sheet
Data update: Aug 20, 2019
Health Report   PCR Protocol   Thawing Method
embryos / sperm >
  Mouse Marking
RBRC No. RBRC05654  
Type TransgeneCartagena  
Species Mus musculus  
Strain name B6.Cg-Tg(CAG-mRFP1,-SOX9,EGFP)1Haak  
Former Common name B6-CAG-SOX9 Tg  
H-2 Haplotype No Data  
Background strain No Data  
 1 Appearance No Data  
Genotype No Data  
Strain development Developed by Haruhiko Akiyama, Kyoto University Hospital in 2007. The mice were crossed to C57BL/6.  
Strain description Sox9, SRY-box containing gene 9 conditional transgenic mice. The transgenes consists of CAG promoter and a floxed monomeric red fluorescence protein 1 (mRFP1) cassette and Sox9-EGFP. Cre expression removes the mRFP1 gene, allowing expression of Sox9 and EGFP protein.  
Colony maintenance Carrier x Noncarrier [C57BL/6NCrSlc]  
Health Report
Gene Details
Promoter No Data  
 1 Symbol IRES  
Symbol name internal ribosomal entry site (EMCV)  
Chromosome UN  
Common name internal ribosomal entry site from EMCV  
Symbol description No Data  
 2 Symbol EGFP  
Symbol name Enhanced Green Fluorescent Protein, F64L and S65T (Jellyfish)  
Chromosome UN  
Common name No Data  
Symbol description No Data  
 3 Symbol HA  
Symbol name human influenza virus HA (hemagglutinin) tag sequence  
Chromosome UN  
Common name No Data  
Symbol description No Data  
 4 Symbol SOX9  
Symbol name SRY-box containing gene 9 (human)  
Chromosome UN  
Common name No Data  
Symbol description No Data  
 5 Symbol Tg(CAG-mRFP1,-SOX9,-EGFP)1Haak  
Symbol name No Data  
Chromosome UN  
Common name No Data  
Symbol description No Data  
 6 Symbol loxP  
Symbol name phage P1 loxP  
Chromosome UN  
Common name No Data  
Symbol description No Data  
Promoter CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)  
 7 Symbol mRFP1  
Symbol name monomeric Red Fluorescent Protein (Discosoma sp.)  
Chromosome UN  
Common name No Data  
Symbol description No Data  
Research applications Cre/loxP system,
Fluorescent Proteins/lacZ System  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
(1) The BIOLOGICAL RESOURCE shall be distributed solely to non-profit organizations for academic research purposes to publish the research results. The BIOLOGICAL RESOURCE must not be used in any for-profit organizations. (2) The RECIPIENT shall obtain prior writen consent to use the BIOLOGICAL RESOURCE from the DEPOSITOR. The consent may be obtainted through internet. (3) After obtaining the consent from the DPOSITOR, the RECIPIENT shall execute the MTA for the use of mRFP 1.0 with Dr. Roger Tsien, UCSD (University of California, San Diego) and send the PDF file of the executed MTA to RIKEN BRC. The form of the MTA may be downloaded at the website of Dr. Tsuen's laboratory ( (4) The RECIPIENT agrees to provide appropriate acknowledgements to the DEPOSITOR and Dr. Roger Tsien in all publications. The RECIPIENT also agrees to cite "J Bone Miner Metab. 2011 29(1):123-9" in all publications. (5) In the event that an invention is derived from the research using BIOLOGICAL RESROUCE, Recipient shall notify the Depositor upon filing a patent application claiming MODIFICATIONS or method(s) of manufacture or use(s) of the BIOLOGICAL RESROUCE. (6) RIKEN BRC shall send the copy of the Material Transfer Agreement execututed between the RECIPIENT and RIKEN BRC to UCSD.
(7) The RECIPIENT agrees to the following terms and condions requested by HHMI(Howard Hughes Medical Institute) and UCSD:The material being transferred to you contains material received from Dr. Roger Tsien, an investigator of the Howard Hughes Medical Institute at the University of California, San Diego ("Tsien material"). The Tsien material is experimental in nature and must be used with prudence and appropriate caution, since not all of its characteristics are known. THE TSIEN MATERIAL IS PROVIDED WITHOUT WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE OR ANY OTHER WARRANTY, EXPRESS OR IMPLIED. THE TSIEN MATERIAL IS PROVIDED WITHOUT REPRESENTATION OR WARRANTY THAT THE USE OF THE TSIEN MATERIAL WILL NOT INFRINGE ANY PATENT OR OTHER PROPRIETARY RIGHT. It cannot be used for any commercial purpose or for work on human subjects, including diagnostic testing. Should the use of this material result in one or more scientific publication(s) you should acknowledge in the paper(s) that the material contained material provided by Dr. Roger Tsien.
The Tsien material must not be distributed to laboratories of for-profit companies. The Tsien material may be distributed to other non-profit laboratories (a) with the prior consent of Dr. Roger Tsien, (b) after an agreement is executed that contains terms substantially similar to the terms set forth herein, including prohibition against further transfers of the Tsien material and use of the Tsien material for commercial purposes or for work on human human subjects, including diagnostic testing, and (c) written notice of the transfer is sent to University of California, San Diego, Technology Transfer and Intellectual Property Services, 9500 Gilman Drive, #0910, La Jolla, CA 92093-0910, Attention: Assistant Vice Chancellor-IP. Written notice may also be emailed to, or faxed to 858-534-7345.  
Additional information
 1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.  
 2 Genotyping protocol <PCR>  
Depositor Akiyama, Haruhiko (Dpertment of Microbiology, Gifu University, School of Medicine)  
Strain Status /
(Expected delivery)

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