Strain Data Sheet

RBRC05655

Strain Information

Image
BRC No.RBRC05655
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129P2-Cbl<tm1(CBL*Q367P)Homy>
Former Common nameCbl Q367P cKI, B6-Cbl/CBL*Q367P KI
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strain
Appearance
Strain developmentDeveloped by Hiroaki Honda, Research Institute for Radiation Biology and Medicine, Hiroshima University in 2012. E14 ES cells (129P2/OlaHsd) were used. The mutant mice were crossed to C57BL/6 mice. Neomycin selection cassette was removed by crossing with FLPe mice.
Strain descriptionExon 5 to 9 of Cbl (Casitas B-lineage lymphoma) gene was replaced with a loxP flanked human CBL, and a human CBL having Q367P variant was inserted the downstream.
Colony maintenanceHeterozygote x Wild-type [C57BL/6NCrlCrlj]
ReferencesBlood, 129, 2148-2160 (2017). 28209720

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
CblCasitas B-lineage lymphoma9CblCasitas B-lineage lymphoma

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
CBLCasitas B-lineage lymphoma(human)9CBL

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site9Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP9loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP9loxP

Ordering Information

Donor DNAphage P1 loxP sites, human CBL cDNA, human CBL<Q367P> cDNA, yeast FRT (flippase recombination target) site, mouse Cbl genomic DNA
Research applicationCre/loxP system
FLP/frt system
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
DepositorHiroaki Honda (Tokyo Women's Medical University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-
Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP (Japanese)

BRC mice in Publications

No Data