Strain Data Sheet
ANIMAL SEARCH SYSTEM
Data update: May 20, 2019
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RBRC No. RBRC05660  
Type TransgeneCartagena  
Species Mus musculus  
Strain name B6.B6D2-Tg(CAG-CAT,spH)5Yyan  
Former Common name CATV-5, B6-CAG-CAT-SpH Tg#5, B6.D2-Tg (CAG-cat, -spH) 5Yyan  
H-2 Haplotype No Data  
Background strain No Data  
Appearance
 1 Appearance No Data  
Genotype No Data  
Strain development Developed by Yuchio Yanagawa, Gunma University Graduate School of Medicine and Hiromu Yawo, Tohoku University Graduate School of Life Sciences in 2002-2003. Transgene was injected into the introduced into the pronuclei of B6D2F1 fertilized eggs. The Tg mice were crossed to C57BL/6J.  
Strain description Vesicular exocytosis at presynaptic terminals is fundamental function for neurotransmission and brain function. The lumen of a secretory vesicle is acidic (pH 5.6), whereas it becomes neutral (pH 7.4) instantaneously upon exocytosis. The intravesicular change of pH can be detected by a fluorescence change of a pH-sensitive derivative of green fluorescent protein (pHluorin). SynaptopHluorin (SpH) is a fusion protein of pHluorin and syntaxin (VAMP2) for targeting to secretory vesicles. CAG-CAT-SpH transgenic mouse expresses SpH under the control of CAG promoter by cre-mediated recombination. This strain is useful for studies of presynaptic terminals in various regions of the brain. Tg mice mice show no obvious abnormality.  
Colony maintenance Carrier x Noncarrier [C57BL/6JJmsSlc]  
Health Report
Gene Details
Promoter No Data  
 1 Symbol Vamp2  
Symbol name vesicle-associated membrane protein 2  
Chromosome UN  
Common name No Data  
Symbol description No Data  
 2 Symbol loxP  
Symbol name phage P1 loxP  
Chromosome UN  
Common name No Data  
Symbol description No Data  
 3 Symbol pHluorin  
Symbol name pH-sensitive green fluorescent proteins, S147E, N149L, I161T, V163A, N164I, K166Q, I167V, R168H, S202H and L231H. (Jellyfish)  
Chromosome UN  
Common name No Data  
Symbol description No Data  
Promoter CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)  
 4 Symbol cat  
Symbol name E. coli Chloramphenicol acetyltransferase (CAT)  
Chromosome UN  
Common name No Data  
Symbol description No Data  
References Unpublished  
Research applications Cre/loxP system,
Fluorescent Proteins/lacZ System,
Neurobiology Research  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Genesis 42, 53-60 (2005).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
1) Any profit organizations cannot use the BIOLOGICAL RESOURCE without contracting license agreements with TOHOKU University.
2) All characteristics of the BIOLOGICAL RESOURCE are not fully known since it was obtained through scientific researches. The recipients should agree that TOHOKU University and the DEPOSITOR will not provide any assurance for the BIOLOGICAL RESOURCE and will not bear any responsibility for the outcome of the use of the BIOLOGICAL RESOURCES without Obligation for Compensation.
3) Either the BIOLOGICAL RESOURCE or the biological materials included in the BIOLOGICAL RESOURCES should not be used in humans or in the edibles creatures even for the diagnosis.
4) The BIOLOGICAL RESOURCE should not be redistributed without the permission by TOHOKU University.  
Additional information
 1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.  
 2 Genotyping protocol <PCR>  
Depositor Yawo, Hiromu (Tohoku University .Life sciences.)  Yawo, Hiromu 
Strain Status /
Availability
(Expected delivery)

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