Strain Data Sheet
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Data update: Apr 19, 2019
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RBRC No. RBRC05702  
Type TransgeneCartagena  
Species Mus musculus  
Strain name C57BL/6J-Tg(CAG-XBP1/luc)23Tiw  
Former Common name ERAI-LUC, B6-ERAI/Luc Tg#23  
H-2 Haplotype No Data  
Background strain C57BL/6JJcl  
Appearance
 1 Appearance No Data  
Genotype No Data  
Strain development Developed by Takao Iwawaki, Advanced Science Institute, RIKEN in 2005. The transgene was injected into the pronuclei of C57BL/6J fertilized eggs. The mice were backcrossed to C57BL/6J.  
Strain description ERAI (ER stress-activated indicator) transgenic mice carry human XBP1 and luciferase fusion gene under control of a CA promoter (cytomegalovirus enhancer and chicken beta-actin promoter). Endoplasmic reticulum (ER) stress is caused by the accumulation of unfolded proteins in the ER lumen, and is associated with vascular and neurodegenerative diseases. The transcripts from ERAI constructs are spliced under ER stress, the spliced mRNA is translated into an XBP1-luc fusion protein, which can be detected by fluorescence of luciferase that mediated by luciferin substrate injection. This strain is useful to monitor physiological and pathological ER stress in vivo.  
Colony maintenance Carrier x Noncarrier [C57BL/6JJcl]  
Health Report
Gene Details
Promoter No Data  
 1 Symbol FLAG  
Symbol name FLAG tag (synthetic)  
Chromosome UN  
Common name No Data  
Symbol description No Data  
 2 Symbol luc  
Symbol name luciferase (firefly)  
Chromosome UN  
Common name No Data  
Symbol description No Data  
Promoter CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)  
 3 Symbol XBP1  
Symbol name X-box binding protein 1  
Chromosome UN  
Common name No Data  
Symbol description No Data  
References   
Research applications Cell Biology Research  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Proc. Natl. Acad. Sci. USA, 106, 16657-16662 (2009).  
Additional information
 1 Mouse of the Month Jan 2013  
 2 Genotyping protocol <PCR>  
Depositor Iwawaki, Takao (Medical research institute,kanazawa medical university)  Iwawaki, Takao 
Strain Status /
Availability
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