Strain Information | |
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Image | |
BRC No. | RBRC05703 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6;129S4-Gt(ROSA)26Sor<tm1.1Tiw> |
Former Common name | ROSA-ERAI-LUCdNeo, B6-ROSA26/ERAI-LUCdNeo |
H-2 Haplotype | |
ES Cell line | RF8 [129S4/SvJae] |
Background strain | |
Appearance | |
Strain development | Developed by Takao Iwawaki, Advanced Science Institute, RIKEN in 2006. RF8 ES cells derived from 129S4/SvJae were used. The neo cassette was removed by crossing with Gt(ROSA)26Sor<tm1(FLP1)Dym> mice. |
Strain description | ERAI (ER stress-activated indicator) knock-in mice. Floxed STOP cassette and modified ERAI construct (HA-2xXBP1deltaDBD(anATG)-LUC-F) were inserted into the Gt(ROSA)26Sor locus. The ERAI expression can be generated by crossing with tissue-specific Cre mice. |
Colony maintenance | Heterozygote x Wild-type [C57BL/6JJcl] |
References | Biochem. J., 425, 117-125 (2009). 19788412 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gt(ROSA)26Sorgene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sor<tm1.1Tiw>targeted mutation 1.1, Takao Iwawaki Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter FLAGFLAG tag (synthetic)6FLAG Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site6Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter HAhuman influenza virus HA (hemagglutinin) tag sequence6HA Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter XBP1X-box binding protein 16XBP1 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP6loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP6loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter lucluciferase (firefly)6luc |
Ordering Information | |
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Donor DNA | adenoviral splice acceptor sequences, Bacteriophage P1 loxP sequence, Yeast FRT (flippase recombination target) site, transcriptional STOP cassette (C-terminal sequence of yeast His3 gene, an SV40 polyA and a 54 splice donor site), influenzavirus HA tag, human XBP1 cDNA, firefly luciferase, FLAG tag, SV40 intron and polyA signal |
Research application | Cell Biology Research Cre/loxP system FLP/frt system Fluorescent Proteins/lacZ System |
Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Biochem. J. 425, 117-125 (2010).The RECIPIENT shall use the BIOLOGICAL RESOURCE in collaboration with the DEPOSITOR (Dr. Takao Iwawaki). |
Depositor | Takao Iwawaki (Gunma University) |
Strain Status | Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- Mouse of the Month Jan 2013 |
BRC mice in Publications |
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No Data |