Strain Information | |
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Image | |
BRC No. | RBRC05704 |
Type | Transgene |
Species | Mus musculus |
Strain name | C57BL/6J-Tg(3xARE/TK-NRF2/luc)M2Tiw |
Former Common name | OKD48-LUC, B6-ARE-NRF2/Luc Tg#M2 |
H-2 Haplotype | |
ES Cell line | |
Background strain | C57BL/6JJcl |
Appearance | |
Strain development | Developed by Takao Iwawaki, Advanced Science Institute, RIKEN in 2010. The transgene was injected into the pronuclei of C57BL/6J fertilized eggs. The mice were backcrossed to C57BL/6J. |
Strain description | Keap1-dependent Oxidative stress Detector transgenic mice, named OKD48 (Keap1-dependent Oxidative stress Detector, No-48). The mice carry human Nrf2 and Flag-tagged luciferase fusion gene under control of 3xARE promoter (oxidative stress inducible promoter). Only cells experiencing oxidative stress can be detected by luciferase luminescence. |
Colony maintenance | Carrier x Noncarrier [C57BL/6JJcl] |
References | Sci. Rep., 2:229 (2012). 22355743 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter FLAGFLAG tag (synthetic)UNFLAG Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter NFE2l2nuclear factor, erythroid derived 2, like 2 (human)UNNFE2l23xARE promoter (mouse anxioxidant/electrophile responsive element, herpes simplex virus TK basal promoter) Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter lucluciferase (firefly)UNluc |
Ordering Information | |
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Donor DNA | 3xARE promoter (mouse anxioxidant/electrophile responsive element, herpes simplex virus TK basal promoter), human Nrf2 cDNA, firefly luciferase cDNA, FLAG tag |
Research application | Cell Biology Research Fluorescent Proteins/lacZ System |
Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Sci. Rep., 2:229 (2012). The RECIPIENT shall use the BIOLOGICAL RESOURCE in collaboration with the DEPOSITOR (Dr. Takao Iwawaki). |
Depositor | Takao Iwawaki (Gunma University) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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Suzuki T, Muramatsu A, Saito R, Iso T, Shibata T, Kuwata K, Kawaguchi SI, Iwawaki T, Adachi S, Suda H, Morita M, Uchida K, Baird L, Yamamoto M. Molecular Mechanism of Cellular Oxidative Stress Sensing by Keap1. Cell Rep 28(3) 746-758.e4(2019) 31315052 |