Strain Data Sheet

RBRC05711

Strain Information

Image
BRC No.RBRC05711
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameSTOCK Tg(tetO-OPN4,mCherry)M5
Former Common nameBitetO-OPN4/mCherry
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Tomomi Tsunematsu, Kenji F. Tanaka Akihiro Yamanaka and Amane Koizumi, National Institute for Physiological Sciences in 2012. The transgene was injected into the pronuclei of CBA/C57BL/6 fertilized eggs. Mixed genetic background.
Strain descriptionBitetO-OPN4/mCherry transgenic mice. These transgenic mice express human OPN4 and mCherry genes under the control of the bi-directional tet-responsive element.
Colony maintenanceCarrier x Carrier [or Crossing to C57BL/6JJcl]
ReferencesNeurosci. Res., 75(1):23-28 (2012). 22868039

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
OPN4opsin 4 (melanopsin)(human)UNOPN4E. coli Tetracycline operator site, Cytomegalovirus CMV minimum promoter

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
mCherryRed Fluorescent Protein (Discosoma sp.)UNmCherry

Ordering Information

Donor DNAE. coli Tetracycline operator site, Human Opn4 gene cDNA, Cytomegalovirus CMV minimum promoter, SV40 PolyA signal, Discosoma mCherry gene cDNA
Research applicationFluorescent Proteins/lacZ System
Neurobiology Research
Tet system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Neurosci. Res., 75(1):23-28 (2012).
DepositorAmane Koizumi (National Institute for Physiological Sciences)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. UCSD MTA
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data