Strain Data Sheet
ANIMAL SEARCH SYSTEM
Data update: Jul 19, 2019
Health Report   PCR Protocol   Thawing Method
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  Mouse Marking
Figure
  Order
RBRC No. RBRC05715  
Type Targeted MutationCartagena  
Species Mus musculus  
Strain name B6D2-Sp56<tm1Osb>  
Former Common name Sp56 KO  
H-2 Haplotype No Data  
ES cell line EGR-G101 [C57BL/6-Tg(CAG/Acr-EGFP)C3-N01-FJ002Osb]  
Background strain Jcl:B6D2F1  
Appearance
 1 Appearance No Data  
Genotype No Data  
Strain development Developed by Yuko Muro and Masaru Okabe, Research Institute for Microbial Diseases, Osaka University in 2009.  
Strain description Sp56 gene knock-out mice. Exon 4 of Sp56 was replaced with a floxed neo cassette.  
Colony maintenance Heterozygote x Wild-type [or Crossing to Jcl:B6D2F1]  
Health Report No Data  
Gene Details
Promoter No Data  
 1 Symbol Zp3r  
Symbol name zona pellucida 3 receptor  
Chromosome 1  
Common name No Data  
Symbol description No Data  
 2 Symbol neo  
Symbol name neomycin resistance gene (E. coli)  
Chromosome No Data  
Common name neo; neomycin;  
Symbol description No Data  
 3 Symbol Frt  
Symbol name yeast FRT (flippase recombination target) site  
Chromosome No Data  
Common name No Data  
Symbol description No Data  
 4 Symbol loxP  
Symbol name phage P1 loxP  
Chromosome No Data  
Common name No Data  
Symbol description No Data  
References   
Research applications Cre/loxP system,
Developmental Biology Research,
FLP/frt system  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Biol. Reprod., 86, 1-6 (2012).
RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University (http://www.uic.osaka-u.ac.jp/en/index.html). The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.  
Additional information
 1 Lab HP  
Depositor Okabe, Masaru (Osaka university)  Okabe, Masaru 
Strain Status /
Availability
(Expected delivery)

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