Strain Data Sheet
Data update: Jun 20, 2019
Health Report   PCR Protocol   Thawing Method
embryos / sperm >
  Mouse Marking
RBRC No. RBRC05753  
Type Targeted MutationCartagena  
Species Mus musculus  
Strain name B6.129S4-Map3k5<tm1Hijo>  
Former Common name ASK1 KO, B6-Map3k5 KO  
H-2 Haplotype No Data  
ES cell line J1 [129S4/SvJae]  
Background strain C57BL/6JJcl  
 1 Appearance No Data  
Genotype No Data  
Strain development Developed by Hidenori Ichijo, Graduate School, Tokyo Medical and Dental University in 2001. J1 ES cells were used. The mutant mice are backcrossed to C57BL/6J.  
Strain description Map3k5 gene knockout mice. The coding region of Exon 1 of Map3k5 gene was replaced with a GFP/PGK-neo cassette (GFP expression was not observed).  
Colony maintenance Homozygote x Homozygote [or Crossing to C57BL/6JJcl] Homozygous mutant mice are viable and fertile.  
Health Report
Gene Details
Promoter No Data  
 1 Symbol Map3k5  
Symbol name mitogen-activated protein kinase kinase kinase 5  
Chromosome 10  
Common name No Data  
Symbol description No Data  
 2 Symbol GFP  
Symbol name Green Fluorescent Protein (Jellyfish)  
Chromosome 10  
Common name No Data  
Symbol description No Data  
 3 Symbol GH  
Symbol name Growth hormone polyA (Bovine)  
Chromosome 10  
Common name No Data  
Symbol description No Data  
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)  
 4 Symbol neo  
Symbol name neomycin resistance gene (E. coli)  
Chromosome 10  
Common name neo; neomycin;  
Symbol description No Data  

Research applications Fluorescent Proteins/lacZ System,
Immunology and Inflammation Research  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use with the results from the use of the BIOLOGICAL RESOURCE.  
Additional information
 1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.  
 2 Mouse of the Month Aug 2014  
 3 Genotyping protocol <PCR>  
Depositor Ichijo, Hidenori (The University of Tokyo)  Ichijo, Hidenori 
Strain Status /
(Expected delivery)

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