Strain Data Sheet
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Data update: Jun 20, 2019
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RBRC No. RBRC05763  
Type Targeted Mutation and TransgeneCartagena  
Species Mus musculus  
Strain name STOCK Izumo1<tm1Osb> Tg(Clgn-Izumo1/mCherry)Osb Tg(CAG/Acr-EGFP)C3-N01-FJ002Osb  
Former Common name Izumo1 KO/Clgn-Izumo1mCherry Tg/CAGAcr-GFP Tg  
H-2 Haplotype No Data  
ES cell line D3 [129S2/SvPas]  
Background strain Jcl:B6D2F1  
Appearance
 1 Appearance No Data  
Genotype No Data  
Strain development Developed by Dr. Naokazu Inoue and Dr. Masaru Okabe, Research Institute for Microbial Diseases, Osaka University in 2005. C57BL/6 and DBA/2 mixed background.  
Strain description Izumo1 knockout and Clgn-Izumo1/mCherry transgene double mutant mice. Izumo1 KO homozygous and Tg positive mice are fertile. The CAG/Acr-GFP transgene is also included.  
Colony maintenance KO : Homozygote x Homozygote ; Clgn-Izumo1/mCherry : Carrier x Noncarrier ; CAG/Acr-GFP : Carrier x Carrier [or Crossing to Jcl:B6D2F1]  
Health Report
Gene Details
Promoter No Data  
 1 Symbol Izumo1  
Symbol name izumo sperm-egg fusion 1  
Chromosome 7  
Common name No Data  
Symbol description No Data  
 2 Symbol mCherry  
Symbol name Red Fluorescent Protein (Discosoma sp.)  
Chromosome UN  
Common name No Data  
Symbol description No Data  
 3 Symbol Frt  
Symbol name yeast FRT (flippase recombination target) site  
Chromosome No Data  
Common name No Data  
Symbol description No Data  
 4 Symbol loxP  
Symbol name phage P1 loxP  
Chromosome No Data  
Common name No Data  
Symbol description No Data  
Promoter CAG promoter (chicken beta-actin promoter, rabbit beta-globin poly A, CMV_IE enhancer), mouse proacrosin promoter, mouse proacrosin signal peptide, acrosin N_terminal peptide  
 5 Symbol GFP  
Symbol name Green Fluorescent Protein (Jellyfish)  
Chromosome UN  
Common name No Data  
Symbol description No Data  
Promoter human PGK promotor, human PGK poly (A)  
 6 Symbol neo  
Symbol name neomycin resistance gene (E. coli)  
Chromosome 7  
Common name neo; neomycin;  
Symbol description No Data  
Promoter mouse calmegin promoter  
 7 Symbol Izumo1  
Symbol name izumo sperm-egg fusion 1  
Chromosome UN  
Common name No Data  
Symbol description No Data  
References   
Research applications Cell Biology Research,
Cre/loxP system,
Dermatology Research,
FLP/frt system,
Fluorescent Proteins/lacZ System  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University (http://www.uic.osaka-u.ac.jp/en/index.html). The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.  
Additional information
 1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.  
 2 Genotyping protocol <PCR>  
Depositor Inoue, Naokazu (Osaka university)  
Strain Status /
Availability
(Expected delivery)

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