Strain Information | |
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Image | |
BRC No. | RBRC05765 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6.Cg-H2az1<tm1.1Hko> H2az2<tm1.1Hko> |
Former Common name | H2AZ1/2 floxed, B6-H2AZ1/2 floxed |
H-2 Haplotype | |
ES Cell line | M1 [(C57BL/6 x 129)F1] |
Background strain | |
Appearance | |
Strain development | Developed by Dr. Haruhiko Koseki, RIKEN Center for Allergy and Immunology in 2009. M1 ES cells derived from (C57BL/6 x 129) F1 were used. The mutant mice were crossed to C57BL/6J. Neomycin resistant genes were removed. |
Strain description | H2A.Z1 and Z2 floxed mice. Histone variant H2A.Z (H2afz) gene is required for mammalian development. |
Colony maintenance | H2afz : Homozygote x Homozygote ; H2afv : Homozygote x Homozygote [or Crossing to C57BL/6JJcl] |
References | Curr. Biol., 11, 1183-1187 (2001). 11516949 |
Health Report | |
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Examination Date / Room / Rack | 2024/02/26Room:4-BRack:E 2023/11/27Room:4-BRack:E 2023/08/30Room:4-BRack:E 2023/08/28Room:4-BRack:E 2023/05/29Room:4-BRack:E 2023/03/22Room:4-BRack:E 2023/02/27Room:4-BRack:E 2022/11/29Room:4-BRack:E |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site11Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP11loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP11loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site3Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP3loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP3loxP |
Ordering Information | |
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Donor DNA | P1 Phage loxP sites, yeast FRT (flipase recombination target) site, mouse H2az1 genomic DNA, mouse H2az2 genomic DNA |
Research application | Cre/loxP system FLP/frt system |
Specific Term and Conditions | The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. |
Depositor | Haruhiko Koseki (RIKEN) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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Lowden C, Boulet A, Boehler NA, Seecharran S, Rios Garcia J, Lowe NJ, Liu J, Ong JLK, Wang W, Ma L, Cheng AH, Senatore A, Monks DA, Liu BH, Leary SC, Cheng HM. Homeostatic control of nuclear-encoded mitochondrial gene expression by the histone variant H2A.Z is essential for neuronal survival. Cell Rep 36(11) 109704(2021) 34525369 Rispal J, Baron L, Beaulieu JF, Chevillard-Briet M, Trouche D, Escaffit F. The H2A.Z histone variant integrates Wnt signaling in intestinal epithelial homeostasis. Nat Commun 10(1) 1827(2019) 31015444 Shen T, Ji F, Wang Y, Lei X, Zhang D, Jiao J. Brain-specific deletion of histone variant H2A.z results in cortical neurogenesis defects and neurodevelopmental disorder. Nucleic Acids Res 46(5) 2290-2307(2018) 29294103 |