Strain Data Sheet
ANIMAL SEARCH SYSTEM
Data update: May 20, 2019
Health Report   PCR Protocol   Thawing Method
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  Mouse Marking
Figure
  Order
RBRC No. RBRC05769  
Type TransgeneCartagena  
Species Mus musculus  
Strain name B6(129S6)-Tg(Aif1-tTA)54Kftnk  
Former Common name Iba1-tTA, B6-Aif1-tTA Tg#54  
H-2 Haplotype No Data  
Background strain No Data  
Appearance
 1 Appearance No Data  
Genotype No Data  
Strain development Developed by Dr. Kenji Tanaka, National Institute for Physiological Sciences. The transgene was injected into the pronuclei of C57BL/6J fertilized eggs. C57BL/6 and 129 mixed background.  
Strain description Iba1-tTA transgenic mice carry a tTA under the control of the modified Iba1 promoter. tTA expression is detected in Iba1 positive macrophage lineage cells (microglia in brain).  
Colony maintenance Carrier x Noncarrier [C57BL/6JJcl]  
Health Report
Gene Details
Promoter No Data  
 1 Symbol GH  
Symbol name Growth hormone polyA (Bovine)  
Chromosome UN  
Common name No Data  
Symbol description No Data  
 2 Symbol Tg(Aif1-tTA)54Kftnk  
Symbol name No Data  
Chromosome UN  
Common name No Data  
Symbol description No Data  
Promoter mouse Iba1 promoter  
 3 Symbol tTA  
Symbol name tTA (E. coli tet repressor (TetR), herpes simplex virus (HSV) VP16 activation domain)  
Chromosome UN  
Common name E. coli tet repressor (TetR), herpes simplex virus (HSV)VP16 activation domain  
Symbol description No Data  
References   
Research applications Neurobiology Research,
Tet system  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Cell Rep., 2, 397-406 (2012).
The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. Prior to requesting the BIOLOGICAL RESOURCE, RECIPIENT must inform the DEPOSITOR of his project using the Approval Form. If the
use of this BIOLOGICAL RESOURCE results in scientific publications, the RECIPIENT should cite Kenji F. Tanaka as co-author in the first paper.  
Additional information
 1 No Data  
 2 Genotyping protocol <PCR>  
Depositor Tanaka, Kenji (Keio University)  Tanaka, Kenji 
Strain Status /
Availability
(Expected delivery)

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