Strain Information | |
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Image | |
BRC No. | RBRC05780 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | C57BL/6N-Rufy3<tm1a(EUCOMM)Hmgu>/B08 |
Former Common name | |
H-2 Haplotype | |
ES Cell line | JM8A3.N1 [C57BL/6N-A<tm1Brd>] |
Background strain | C57BL/6NTac |
Appearance | |
Strain development | |
Strain description | |
Colony maintenance | |
References |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Rufy3RUN and FYVE domain containing 35Rufy3<tm1a(EUCOMM)Hmgu>targeted mutation 1a, Helmholtz Zentrum Muenchen GmbH Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site5Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site5Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Iresinternal ribosomal entry site (EMCV)5 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SASplice acceptor5SA Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter lacZbeta-galactosidase (E. coli)5 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP5loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP5loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP5loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)5hBactP (human beta actin promoter) Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SV40 polyA signal5 |
Ordering Information | |
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Donor DNA | yeast FRT (flipase recombination target) sites, En2 SA (mouse En2 intron 2/exon 3 splice acceptor sequence), Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), E. coli lacZ, SV40 polyadenylation site, phage P1 loxP sites, hBactP (human beta actin promoter), E. coli neo, mouse Rufy3 genomic DNA |
Research application | Cre/loxP system FLP/frt system Fluorescent Proteins/lacZ System |
Specific Term and Conditions | The RECIPIENT should acknowledge the EUCOMM, RIKEN BRC and the International Mouse Phenotyping Consortium as the original creator of the targeted mouse ES cells and the provider of the knockout mice, respectively. |
Depositor | RIKEN BRC (RIKEN BRC) |
Strain Status | Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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Hertz NT, Adams EL, Weber RA, Shen RJ, O'Rourke MK, Simon DJ, Zebroski H, Olsen O, Morgan CW, Mileur TR, Hitchcock AM, Sinnott Armstrong NA, Wainberg M, Bassik MC, Molina H, Wells JA, Tessier-Lavigne M. Neuronally Enriched RUFY3 Is Required for Caspase-Mediated Axon Degeneration. Neuron 103(3) 412-422.e4(2019) 31221560 |