Strain Data Sheet

RBRC05829

Strain Information

Image
BRC No.RBRC05829
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6J-Tg(hSMA-rtTA)1
Former Common nameHSMA-rtTA-C, B6-SMA-rtTA Tg#1
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Dr. Takahiro Iwamoto, School of Medicine, Fukuoka University in 2009. C57BL/6J background.
Strain descriptionThese transgenic mice express a rtTA gene under the control of the human SMA gene promoter.
Colony maintenanceCarrier x Noncarrier [C57BL/6JJmsSlc]Tg mice are viable and fertile.
References

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
rtTAE. coli Reverse tetracycline transactivator (Mutated tetrepressor DNA-binding domain and the transactivation domain of VP-16)UNrtTAE. coli tet repressor (TetR), herpes simplex virus (HSV)VP16 activation domainhuman smooth muscle alpha-actin promoter

Ordering Information

Donor DNAHuman smooth muscle alpha-actin promoter, HSV rtTA-Advanced, SV40 polyA signal
Research applicationGenetics Research
Tet system
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. Before the first publication on the BIOLOGICAL RESOURCE by DEPOSITOR, the RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR (principal investigator).
DepositorTakahiro Iwamoto (Fukuoka University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data