Strain Data Sheet
ANIMAL SEARCH SYSTEM
Data update: Aug 20, 2019
Health Report   PCR Protocol   Thawing Method
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  Mouse Marking
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RBRC No. RBRC05843  
Type Targeted MutationCartagena  
Species Mus musculus  
Strain name B6.Cg-Actb<tm1(tetO-ChR2*E123T/T159C)Ahky>  
Former Common name TetO ET/TC, B6-tetO-ChR2/ETTC KI  
H-2 Haplotype No Data  
ES cell line G0G1  
Background strain No Data  
Appearance
 1 Appearance No Data  
Genotype No Data  
Strain development Dr. Akihiro Yamanaka, National Institute for Physiological Sciences in 2012.  
Strain description The tetO-ChR2(E123T/T159C) cassette was inserted into the Actb gene.  
Colony maintenance Homozygote x Homozygote [or Crossing to C57BL/6JJcl]
Homozygous mice are viable and fertile.  
Health Report
Gene Details
Promoter No Data  
 1 Symbol Actb  
Symbol name actin, beta  
Chromosome 5  
Common name No Data  
Symbol description No Data  
 2 Symbol EYFP  
Symbol name Enhanced Yellow Fluorescent Protein (Jellyfish)  
Chromosome 5  
Common name No Data  
Symbol description No Data  
 3 Symbol Frt  
Symbol name yeast FRT (flippase recombination target) site  
Chromosome 5  
Common name No Data  
Symbol description No Data  
 4 Symbol HBB2  
Symbol name rabbit beta-globin intron  
Chromosome 5  
Common name No Data  
Symbol description No Data  
 5 Symbol loxP  
Symbol name phage P1 loxP  
Chromosome 5  
Common name No Data  
Symbol description No Data  
 6 Symbol polyA  
Symbol name SV40 polyA  
Chromosome 5  
Common name No Data  
Symbol description No Data  
Promoter E. coli Tetracycline operator, CMV minimal promotor, rabbit Beta globin intron  
 7 Symbol ChR2  
Symbol name C. reinhardtii Channelrhodopsin2  
Chromosome 5  
Common name No Data  
Symbol description No Data  
References   
Research applications Cre/loxP system,
FLP/frt system,
Fluorescent Proteins/lacZ System,
Tet system  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.  
Additional information
 1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.  
 2 Mouse of the Month Dec 2013  
 3 Genotyping protocol <PCR>  
Depositor Yamanaka, Akihiro (Research Institute of Environmental Medicine Nagoya university)  Yamanaka, Akihiro 
Strain Status /
Availability
(Expected delivery)

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